濒危树种金钱松RAPD体系的建立和遗传多样性分析

RAPD analysis of genetic diversity for Pseudolarix amabilis:a critically endangered plant

通过优化随机扩增多态性DNA(RAPD)反应体系,对浙江省不同来源的金钱松Pseudolarix amabilis进行遗传多样性分析。结果表明:18条随机引物在62个样品中可检测到172个可重复位点,其中多态性位点有170个,多态性比率为99.2%。聚类分析的结果表明:同一来源金钱松材料聚为一类,说明金钱松天然群体的RAPD多态性分类和其地理分布有一定的关系,但也有特殊个体不一致,表明金钱松天然种群体存在较高的遗传多样性,并建议对金钱松进行就地保存和迁地保存。

In order to protect germplasm resources of endangered plant： Pseudolarix amabilis, genetic diversity of Pseudolarix amabilis from different sources in Zhejiang Province were analyzed using an optimal protocol of random amplified polymorphism DNA （RAPD） followed by a cluster analysis in this study. Results showed that for 62 individuals with 18 random primers, 172 repetitive loci were detected, among which 170 were polymorphic for a species level polymorphic loci rate of 99.2%. The cluster analysis showed that materials from the same sources were classified together. Thus, RAPD polymorphism classification for natural populations of P. amabilis was mostly related to geographic distribution, genetic diversity was high among the P. amabilis population, and both in situ preservation and ex-situ conservation were possible. [Ch, 2 fig. 3 tab. 31 ref.]