三七总皂苷对海水淹溺兔肺泡巨噬细胞TNF-α mRNA表达的影响

Effects of Panax Notoginseng Saponins on Expression of TNF-α mRNA in Alveolar Macrophage of Rabbits with Sea Water Drowning

目的观察海水淹溺后家兔肺组织光镜下变化,并探讨三七总皂苷干预对海水淹溺后家兔肺泡巨噬细胞内TNF-αmRNA表达的影响。方法采用气管切开插入塑料导管、向气管内灌海水4mL/kg,双肺自主通气的方法模拟海水淹溺造成急性肺损伤模型,随机分为对照组、海水淹溺组、三七总皂苷治疗组。于淹溺后进行血气分析,采集支气管肺泡灌洗液,分离和培养肺泡巨噬细胞,并采集肺组织进行病理学分析,逆转录-聚合酶链反应(RT-PCR)检测肺泡巨噬细胞中TNF-αmRNA的表达。结果光镜下,海水淹溺兔肺组织炎症细胞大量浸润,经三七总皂苷治疗后肺组织损伤程度减轻。RT-PCR分析示淹溺后TNF-αmRNA表达显著增高,90min后达最高峰,而三七总皂苷可使之降低。结论海水淹溺急性肺损伤可能与肺泡巨噬细胞内TNF-αmRNA高表达有关。三七总皂苷可降低TNF-αmRNA的高表达,减轻肺损伤程度。

Objective：To observe the histopathology changes of rabbits drowned by sea water and to explore the effect of panax notoginseng saponins on TNF-α mRNA expression.Methods：The drowning model was established with inserting plastic tube to the trachea of rabbits and sea water（4mL/ kg） was poured into air tube with the both of lung auto ventilated to simulate the process of drowning.Rabbits were randomly divided into the control group,the drowning group and the panax notoginseng saponins group.Then the expression of TNF-α mRNA in the alveolar macrophage of rabbits was determined by reverse-transcription polymerase chain reaction（RT-PCR） after drawing.The infiltration of PMN was examined by histopathologic,while the blood gas analysis was determinated.Results：After drowning,the infiltration of PMN and the expression of TNF-α mRNA were significantly increased.However,Panax notoginseng saponins could inhibit the expression in TG and extenuate the degree of acute lung injury.Conclusion：For sea water respiratory distress syndrome,the acute lung damage at early stage might be related to the high expression of TNF-α mRNA in alveolar macrophage.That the panax notoginseng saponins can significantly decrease the expression of TNF-α mRNA in the alveolar macrophage could lighten the degree of acute lung injury.