体外药动模型中强力霉素对嗜水气单胞菌的药效研究

Pharmacodynamic effect of doxycycline on Aeromonas hydrophila in vitro pharmacokinetic model

为保证强力霉素(doxycyline)在防治水产动物疾病上的合理应用,通过建立强力霉素体外药动-药效同步(PK-PD)模型的方法,研究了强力霉素的药物动力学和抑制嗜水气单胞菌(Aeromonas hydrophila)的药效学之间的关系。结果显示,强力霉素对嗜水气单胞菌的最小抑菌浓度(M IC)和最小杀菌浓度(MBC)分别为2.0μg/mL和8.0μg/mL。在消除半衰期为38.61 h的模型内,2MIC的强力霉素对嗜水气单胞菌仅能抑制3 h,模型运行3 h后细菌出现再生长。4、8和16M IC的强力霉素对嗜水气单胞菌能够起到持续的抑制作用。结果表明,强力霉素对嗜水气单胞菌的药效与药物浓度关系密切。当Cmax/M IC>3.96时,强力霉素能够对嗜水气单胞菌起到持续的抑制作用。

For the rational usage of doxycycline on fish,an in vitro pharmacokinetics-pharmaco-dynamics model of doxycycline was developed,and the pharmacokinetics-pharmacodynamics relationship of doxycycline on Aeromonas hydrophila was described by the model.In consequence,the accurate MIC and MBC of doxycycline on Aeromonas hydrophila were 2.0 μg/mL and 8.0 μg/mL.In the in vitro model with elimination half-life of 38.61 h,A.hydrophila was inhibited for only 3 h by 2 MIC doxycycline,and A.hydrophila regrew after the model worked about 3 h.4,8 and 16 MIC doxycycline showed continously inhibitive action on A.hydrophila.It concluded that the inhibiting effect of doxycycline against A.hydrophila was closely related to its concentration,and continous inhabitation could be achieved at the condition of Cmax/MIC3.96.