基于酪氨酸酶-氧化石墨烯的生物传感器的制备及应用

Fabrication of Biosensor Based on Tyrosinase-Graphene Oxide and Its Application

研制了基于酪氨酸酶和氧化石墨烯修饰丝网印刷电极的生物传感器,并用于水中邻苯二酚的测定.1-芘丁酸琥珀酰亚胺酯分子通过非共价的π-π堆积作用吸附到氧化石墨烯上,酪氨酸酶与1-芘丁酸琥珀酰亚胺酯形成酰胺键,组装到氧化石墨烯上,制得纳米复合材料.该材料为酪氨酸酶的固定提供了有利的微环境,并且能很好地保持其生物活性.传感器对邻苯二酚响应灵敏、快速,达到稳态电流时间在9 s以内.实验结果表明,传感器的重现性、选择性和稳定性良好,检测线性范围为8.1×10-8 mol/L～2.2×10-5 mol/L,检出限为4.7×10-8 mol/L.所研制的传感器可应用在水中酚类化合物的现场快速检测之中.

A disposable biosensor has been developed for the determination of catechol in water based on tyrosinase-graphene oxide modified screen printed electrode. Graphene oxide sheets （GS） were noncovalently-functionalized with 1- pyrenebutanoic acid and succinimidyl ester （PANHS）. Subsequently, the tyrosinase could covalently bind with PANHS- functionalized GS by forming amide bond. The as-prepared biocompatible nanoeomposite could provide a biocompatible mieroenvironment to protect the activity of tyrosinase effectively. This disposable biosensor exhibited a fast amperometrie response （ less than 9 s） , and a high sensitivity for monitoring cateehol with good repeatability, selectivity and stability. The method showed good linearity in the range from 8. 1×10-8mol/L～2.2×10-5mol/L catechol with a detection limit of 4. 7 ×10-8mol/L. The results showed that the as-prepared disposable biosensor could be used in the rapid and in-situ monitoring of phenolic compounds in water samples.