一个豇豆上的番茄黄化曲叶病毒分离物的分子鉴定及其基因组全序列(英文)

Molecular Identification and Complete Nucleotide Sequence of a Tomato Yellow Leaf Curl Virus Isolated from Vigna sinensis

2008年秋,在上海郊区大棚栽培的豇豆上发现一种叶片褪绿和黄化病害症状。通过PCR从感病的植株叶片中扩增出番茄黄化曲叶病毒的全序列。序列比较及系统进化分析表明,这种病毒是菜豆金色黄花叶病毒属成员之一的Tomato Yellow Leaf Curl Virus-Israel(TYLCV-IL)。基因组是由2781个核苷酸组成的环状单链DNA,具有典型的旧世界单组分菜豆金色黄花叶病毒属病毒的特征,有6个开放阅读框,V2和V1(外壳蛋白基因)在病毒链上,而C3、C2、C1和C4在互补链上。这是国内番茄黄化曲叶病毒侵染豇豆的首次报道。

In the fall of 2008,the cowpea growing in a greenhouse in Shanghai suburban suffered a disease characterized by chlorotic and yellowing leaves. The complete nucleotide sequence of a tomato yellow leaf curl virus was amplified from DNA extracted from diseased plants. The sequence comparisons and phylogenetic analysis revealed that the pathogen was Tomato yellow leaf curl virus-Israel（TYLCV-IL）,a member of the genus Begomovirus.