合欢茎叶提取物对8种植物病原真菌抑菌活性的研究

Research on Antifungal Activity of Extract from the Stems and Leaves of Albizia julibrissin Against 8 Kinds of Plant Pathogens

以8种植物病原真菌为供试菌,采用生长速率法测定了合欢茎叶提取物的抑菌活性。结果表明,在供试浓度为3mg/mL时,合欢茎叶提取物对棉花枯萎病菌(Fusarium oxysporumf.sp.vasinfectum)、终极腐霉病菌(Pythium ultimum)、棉花立枯病菌(Rhizoctonia solani)的活性较高,抑菌率分别为42.77%、72.29%、46.34%;其EC50分别为3.982 8、1.036 1、3.493 1mg/mL。合欢茎叶提取物的不同极性溶液萃取物中正丁醇萃取物对终极腐霉病菌的抑菌活性较高,其EC50为0.884 1mg/mL。针对正丁醇萃取物进行柱层析,浓度为1mg/mL时,组分4对终极腐霉有较高的生物活性,抑菌率为83.25%。因此,对组分4的分离纯化是下一步研究的重点。

Based on growth rate methods, stem and leaf extract of Albizia julibrissin was tested for its antifungal activity against 8 kinds of plant pathogens. Results showed that extract from the stems and leaves of A. julibrissin had strong antifungal activity against Fusariurn occysporum f. sp. vasinfectum, Pythium ultimura and Rhizoctonia solani. The inhibition rates were 42. 77%,72. 29%and 46.34% at the concentration of 3 mg/mL,and their EC50 were 3. 982 8,1. 036 1,3. 493 1 mg/mL, respectively. The extract was fractionated using different polarity solvents. The fraction with solvent n-butyl alcohol had the highest antifungal activity against P. ultimurn and its EC50 was 0. 884 1 mg/mL. The n-butyl alcohol fraction was separated by silica gel column chromatograghy. The fraction 4 had stronger activity against the P. ultimum with 83.25% of the inhibition rate at the concentration of 1 mg/mL. Our further study interest will focus on the separation and purification of fraction 4 from stems and leaves of Albizia julibrissin.