全内反射荧光光谱在蛋白质材料相互作用领域的应用(英文)

Application of Total Internal Reflection Fluorescence in Protein-Material Interactions Fields

蛋白质表面相互作用对药物释放、生物传感器、亲和色谱和离子色谱以及仿生材料在生物环境中均起到关键作用。许多技术可用来研究不同材料界面间的蛋白质密度、结构和取向。全内反射技术由于具有快速、无损、灵敏以及灵活等特点,在研究分子水平蛋白质界面耦合过程发挥着重要的作用。描述了全内反射的原理、技术以及在检测和控制生物分子间作用等方面的应用,主要包括蛋白质—蛋白质,蛋白质-DNA,DNA-DNA以及蛋白质—膜等界面体系。这些研究有助于进一步加强对蛋白质表面相互作用的理解。近年来全内反射技术在蛋白质领域的应用,为其在生物物理和生物化学方面的应用提供了依据和可能。

Protein-surface interactions play a significant role in drug delivery,biosensor technology,affinity or ion exchange chromatography and artificial materials under a biological environment.Many elaborate techniques have been applied for the investigation of protein density,structure or orientation at the interfaces.One particularly useful technique for studying protein surface-associated processes at the molecular level is total internal reflection fluorescence（TIRF）,which is fast,non-destructive,sensitive and versatile technique.In this paper,the principles and techniques of TIRF were described and the broad range of TIRF and TIRF-electrochemical systems for detection and control of biomolecular interaction including protein-protein,protein-DNA,DNA-DNA,protein-membrane is summarized.These studies are providing enhanced understanding of protein-surface interaction.Several recent developments in TIRF from protein-material fields are likely to find future application in other biophysics and biochemistry.