摘要
分别对1 g重力和模拟微重力条件下成熟的昆明小鼠卵母细胞进行体外受精试验,研究模拟微重力条件下成熟卵母细胞的受精能力,探讨模拟微重力对卵母细胞成熟的影响。结果显示,1 g重力条件下成熟的小鼠卵母细胞的体外受精率为35.82%,显著高于模拟微重力条件下成熟的卵母细胞的体外受精率。可见,模拟微重力不利于卵母细胞的体外成熟,模拟微重力损伤卵母细胞的受精能力。
Mouse oocytes were cultured in microgravity simulated by a rotating wall vessel bioreactor and in Ig gravity in vitro, respectively. We examined the fertilization rate of the mouse ooeytes matured in different gravity conditions. The fertilization rate of mouse oocytes matured in lg gravity was 35.82%, and the oocytes matured in simulated microgravity could not be fertilized in vitro. The results suggest that mouse oocytes maturation and fertilization ability were compromised by the simulated mierogravity in vitro.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2011年第5期694-697,共4页
Journal of Anhui Agricultural University
基金
国家973计划(2011CB944202
2010CB945001)
国家转基因生物新品种培育重大专项(2008zx08006-004)共同资助
关键词
模拟微重力
卵母细胞
受精
simulated microgravity
oocytes
fertilization