茶树上冰核活性细菌的分离、鉴定及防治

Identification and control of one strain of ice nucleation active(INA) bacteria isolated from the tea plant

霜冻发生时从茶树上分离到1株冰核活性细菌,该菌株在﹣5℃下在2 min内冻滴率达到85%,一个冰核产生需要的细胞数约为5.4×103个,该菌株具有明显冰核活性。对该菌株进行16S rDNA序列对比分析,判断该菌株为萎蔫短小杆菌(Curtobacterium flaccumfaciens)。在-5℃条件下,药剂防治效率顺序为:次氯酸钙>Tween80>藏红>亚甲蓝。在同一生态条件下的土壤中分离出1株拮抗菌,经鉴定该菌株为黄孢原毛平革菌(Phanerochaete chrysosporium)。

One of ice nucleation active（INA） bacteria was isolated from the tea plant when frost occurred,and the freezing percentage of this strain was 85% at-5℃ in 2 min.The cells/ice nuclei were about 5.4×103,and the strain shows significant ice nucleation activity.After 16S rDNA sequences of the isolates in GenBank were analyzed,the strain was identified as Curtobacterium flaccumfaciens.The efficiency of chemical control order at ﹣5℃ was calcium hypochlorite〉 Tween 80〉 saffron〉 methylene blue.Under the same ecological condition,an-tagonist bacterium was isolated from the soil,which was identified as Phanerochaete chrysosporium.