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LINE-1 ORF-1p过表达对肝癌细胞株SMMC7721增殖和锚定非依赖性生长的影响 被引量：5

Effects of LINE-1 ORF-1p overexpression on proliferation and anchor-independent growth of SMMC7721 hepatoma cell line
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摘要 目的研究逆转座子L1编码蛋白ORF-1p的表达对肝癌细胞株SMMC7721细胞增殖的影响。方法利用带Flag标签的L1 ORF1表达载体,转染SMMC7721细胞;采用Western blot法检测反转座子L1 ORF1编码蛋白ORF-1p的表达;采用软琼脂成集落实验和相关报告基因检测ORF-1p表达对肝癌细胞p53、p15和p21活性的影响。结果在SMMC7721细胞中,Flag-ORF-1p表达能够显著促进该细胞的增殖(P<0.05),增强该细胞的锚定非依赖性生长(P<0.05),降低p53、p15和p21报告基因的活性。结论 L1基因编码蛋白ORF-1p能够促进肝癌细胞的生长、浸润以及肿瘤的形成。 Objective To investigate the effects of L1 encoded ORF-1p on the proliferation of SMMC7721 cells.Methods Recombinant expression vector pcDNA3.0-Flag-ORF1 was used to transfect SMMC7721 cells;Expression of ORF1p in the transfected cells was detected by Western blotting;Proliferation and anchor-independent growth of the cells transfected with Flag-ORF1p vector were detected by MTT assays and soft-agar anchored colony formation assays;Transcriptional activity of p15,p21 and p53 reporter genes was analyzed by luciferase reporter system.Results Flag-ORF-1p could enhance the proliferation and anchor-independent growth ability of SMMC7721 cells;It also reduced the transcriptional activity of p15,p21 and p53 luciferase report genes.Conclusion ORF-1p of L1 might enhance the SMMC7721 cell proliferation and anchor-independent growth through regulation of some genes.
作者 高旭东 陆荫英 冯帆 王春平 王鈜 常秀娟 杨永平
机构地区 解放军第 解放军军事医学科学院毒物药物研究所
出处 《实用肝脏病杂志》 CAS 2011年第5期323-326,共4页 Journal of Practical Hepatology
基金 国家科技重大专项课题(2008ZX100022018) 首都医学发展基金课题(2009-2041)
关键词 肝癌细胞株SMMC7721 逆转座子L1 开放读码框-1p 蛋白质转移吸印技术 SMMC7721 cells Retrotransposon LINE-1 Open reading frame-1p Carcinogenesis
分类号 R735.7 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1ROMAN-GOMEZ J,JIMENEZ-VELASCO A,AGIRRE X,et al.Promoter hypomethylation of the LINE-1 retrotransposable elements activates sense/antisense transcription and marks the progression of chronic myeloid leukemia[J].Oncogene,2005,24:7213-7223.
  • 2GOODIER JL,ZHANG L,VETTER MR,et al.LINE-1 ORF1 protein localizes in stress granules with other RNA-binding proteins,including components of RNA interference RNA-induced silencing complex[J].Mol Cell Biol,2007,27:6469-6483.
  • 3PORNTHANAKASEM W,KONGRUTTANACHOK N,PHUANGPHAIROJ C,et al.LINE-1 methylation status of endogenous DNA double-strand breaks[J].Nucleic Acids Res,2008,36(11):3667-3675.
  • 4GASIOR SL,WAKEMAN TP,XU B,et al.The human LINE-1 retrotransposon creates DNA double-strand breaks[J].J Mol Biol,2006,357:1383-1393.
  • 5XING J,ZHANG Y,HAN K,et al.Mobile elements create structural variation:analysis of a complete human genome[J].Genome Res,2009,19:1516-1526.
  • 6ESTECIO MR,GHARIBYAN V,SHEN L,et al.LINE-1 hypomethylation in cancer is highly variable and inversely correlated with microsatellite instability[J].PLoS ONE,2007,2(5):e399.
  • 7SINIBALDI-VALLEBONA P,LAVIA P,GARACI E,et al.A role for endogenous reverse transcriptase in tumorigenesis and as a target in differentiating cancer therapy[J].Genes Chromosomes Cancer,2006,45(1):1-10.
  • 8MARTIN SL.The ORF1 protein encoded by LINE-1:structure and function during L1 retrotransposition[J].J Biomed Biotechnol,2006,2006(1):45621.
  • 9高旭东,胡明明,朱运峰.下调LINE-1编码蛋白ORF-1p对肺癌细胞A549生物学特征的影响[J].中国生物工程杂志,2010,30(6):14-20. 被引量：7
  • 10BUNZ F,DUTRIAUX A,LENGAUER C,et al.Requirement for p53 and p21 to sustain G2 arrest after DNA damage[J].Science,1998,282(5393):1497-1501.

二级参考文献19

  • 1Narita N, Nishio H, Kitoh Y, et al. Insertion of a 5truncated L1 element into the 3'end of exon 44 of the dystrophin gene resulted in skipping of the exon during splicing in a case of Duchenne muscular dystrophy. J Clin Invest, 1993, 91 : 1862-1867.
  • 2Bailey J A, Carrel L, Chakravarti A, et al. Molecular evidence for a relationship between LINE-1 elements and X chromosome inactivation: the Lyon repeat hypothesis. Proc Natl Acad Sci USA, 2000, 97:6634-6639.
  • 3Allen E, Horvath S, Tong F, et al. High concentrations of long interspersed nuclear element sequenee distinguish monoallelically expressed genes. Proc Natl Acad Sci USA, 2003, 100:9940-9945.
  • 4Burwinkel B, Kilimann M W. Unequal homologous recombination between LINE-1 elements as a mutational mechanism in human genetic disease. J Mol Biol, 1998, 277:513-517.
  • 5Liu L, Chen X H, Huang J, et al. NSSRI promotes neuronal differentiation of mouse embryonic carcinoma P19 cells. Neuroreport, 2004, 15:823-828.
  • 6Anthony V Furanol, David D Duvernell, Stephane Boissinot. L1 (LINE-1) retrotransposon diversity differs dramatically between mammals and fish. Trends in Genetics ,2004 ,20 ( 1 ) :9-14.
  • 7Xi Shi, Andrei Seluanov, Vera Gorbunova. Cell divisions are required for L1 retrotransposition. Molecular and Cellular Biology, 2007, 27(4 ) :1264-1270.
  • 8Roman-Gomez J, Jimenez-Velasco A, Agirre X, et al. Promoter hypomethylation of the LINE-1 retrotransposable elements activates sense/antisense transcription and marks the progression of chronic myeloid leukemia. Oncogene ,2005,24:7213-7223.
  • 9Goodier J L, Zhang L, Vetter M R, et al. LINE-10RF1 protein localizes in stress granules with other RNA-binding proteins, including components of RNA interference RNA-induced silencing complex. Mol Cell Biol,2007,27:6469-6483.
  • 10Wichai Pornthanakasem, Narisom Kongruttanachok, Chutipa Phuangphairoj. LINE-1 methylation status of endogenous DNA double-strand breaks.. Nucleic Acids Research, 2008, 6 ( 11 ) : 36673675.

共引文献6

同被引文献43

  • 1Fan Feng,Yin-Ying Lu,Fan Zhang,Xu-Dong Gao,Chuan-Fu Zhang,Alex Meredith,Zhong-Xian Xu,Yu-Tao Yang,Xiu-Juan Chang,Hong Wang,Jian-Hui Qu,Zhen Zeng,Jun-Lan Yang,Chun-Ping Wang,Yun-Feng Zhu,Jia-Jun Cui,Yong-Ping Yang.Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma[J].World Journal of Gastroenterology,2013,19(7):1068-1078. 被引量：8
  • 2Goodier JL,Zhang L,Vetter MR,etal.LINE-1 ORF1 proteinlocalizes in stress granules with other RNA-binding proteins,including components of RNA interference RNA-inducedsilencing complex[J].Mol Cell Biol,2007,27(18):6469-6483.
  • 3Estécio MR,Gharibyan V,Shen L,et al.LINE-1 hypo-methylation in cancer is highly variable and inversely correlatedwith microsatellite instability[J].PLoS One,2007,2(5):e399.
  • 4Brummelkamp TR,Bernards R,Agami R.A system for stableexpression of short interfering RNAs in mammalian cells[J].Science,2002,296(5567):550-553.
  • 5Roman-gomez J, Jimenez-velasco A, Agirre X, et al. Promoterhypomethylation of the line-1 retrotransposable elementsactivates sense/an tiseuse transcription and marks the progression of chronic myeloid leukemia [ J ] . Oncogene, 2005, 24 ( 48 ) : 7213-7223.
  • 6Goodier JL, Zhang L, etter MR, et al. Line-10RF1 protein localizes in stress granules with other RNA-binding proteins, including components of RNA interference RNA-induced silencing complex [ J ] . Mol Cell Biol, 2007, 27 ( 18 ) : 6469-6483.
  • 7Pornthanakasem W, Kongruttanachok N, Phuangphairoj C, et al. Line-1 methylation status of endogenous DNA double-strand breaks [ J ] . Nucleic Acids Res, 2008, 36 ( 11 ) : 3667-3675.
  • 8Gasior SL, Wakeman TP, Xu B, et ul. The human line-1 retrotransposon creates DNA double-strand breaks [ J ] . J Mol Biol, 2006, 357 ( 5 ) : 1383-1393.
  • 9Xing J, Zhang Y, Han K, et al. Mobile elements create structural variation : analysis of a complete human genome [ J ] . Genome Res, 2009, 19 ( 9 ) : 1516-1526.
  • 10Est e cio MR, Gharibyan V, Shen L, et al. LINE-I hypomethylation in cancer is highly variable and inversely correlated with microsatellite instability [ J ] . PLoS One, 2007, 2 ( 5 ) : e399.

引证文献5

二级引证文献16

实用肝脏病杂志
2011年 第5期

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