siRNA阻抑Survivin基因表达对肝癌细胞周期的影响

Research progression of effects on hepatocarcinoma cell cycle by inhibition of survivin gene by siRNA

应用RNA干扰技术(RNAi)对siRNA抑制肝癌细胞株内源Survivin基因的表达进行研究。笔者通过Western blot和半定量RT-PCR技术来检测Survivin蛋白的表达和mRNA转录水平的变化,观察小分子干扰RNA(siRNA)对肝癌细胞细胞周期的影响,并对RNAi技术的作用机制及在肿瘤基因治疗方面的实验研究做一综述。

To apply the small interfering RNA targeting suivivin to inhibit expression of endogenous surviving gene in human hepatocarcinoma cell.The expression level of surviving was determined by Western blot and the transcription of survivin gene was detected by semi-quantitative RT-PCR.To investigate the effects on cell cycle of hepatocellular carcinoma cells by small interfering RNA.The small interfering RNA is more precise and effective and has much value of clinical application than antisense oligonucleotides by inhibiting target gene.This paper mainly deals with the mechanism of action of RNAi and the progresses in empirical study on the gene therapy.