转录因子E2F-1与垂体肿瘤转化基因(PTTG)在大鼠PRL瘤中的表达

Expression of Transcription Factor E2F-1 and Pituitary Tumor Transforming Gene(PTTG) in Rat Prolactinoma

目的通过检测垂体肿瘤转化基因(PTTG)与腺病毒E2启动子结合因子1(E2F-1)在大鼠催乳素(PRL)瘤中的表达来探讨两者在PRL瘤发生发展过程中的作用。方法 40只大鼠随机分为两组:实验组(E组,n=20):皮下植入17β-雌二醇的方法诱发大鼠PRL瘤;对照组(C组,n=20):皮下植入空白硅胶管。雌激素诱导10周后处死大鼠,心脏穿刺取血,4%多聚甲醛体内灌流取出脑垂体,称重,ELISA方法检测两组大鼠血清PRL水平,垂体组织行病理组织学观察,免疫组化SP方法检测两组大鼠垂体组织中PTTG蛋白质、E2F-1蛋白质的表达。结果雌二醇作用10周后,据垂体重量、垂体组织学变化和血清PRL水平证实PRL瘤诱导成功。PRL瘤组中,PTTG蛋白质、E2F-1蛋白质均明显高于对照组,差异具有统计学意义(P<0.01);且PTTG蛋白质和E2F-1蛋白质的表达呈明显正相关(γ=0.764,P<0.01)。结论 PTTG与E2F-1在大鼠PRL瘤中共同过度表达,参与了大鼠PRL瘤的发生发展。

Objective To detect the expression of adenovirus E2 promoter binding factor 1（E2F-1）and Pituitary tumor transforming gene（PTTG） in rat prolactinoma and to explore their role in the development of prolactinoma.Methods 40 male Wistar（120-150g） rats were randomly divided into two groups： experimental group（E group,n=20）： 17β-estradiol stuffed in silicone tube was subcutaneously implanted to induce rat prolactinoma model;the control group（C group,n=20） blank silicone tube was subcutaneously implanted.After 10 weeks,all the rats were executed,blood was taken by cardiac puncture,4% paraformaldehyde was intracardially perfused and pituitary tissues were harvested,weighed.Serum PRL levels were detected by ELISA method;Pituitary tissue histopathological changes were observed;both expression of PTTG protein and E2F-1 protein were detected by immunohistochemistry SP method.Results Prolactinoma models were induced successfully as reflected by pituitary weight 、pituitary pathological change and serum PRL level.The expression of PTTG protein and E2F-1 protein in prolactinoma group was obviously higher than those in control group,（P〈0.01） and PTTG protein expression was positively correlated with E2F-1 protein（γ=0.764,P〈0.01）.Conclusion PTTG and E2F-1 were co-overexpressed in prolactinoma,indicating their role in the development of prolactinoma.