环孢素A对异种脱细胞神经移植的影响

The Effect of Cyclosporin A on Acellular Heterogeneous Nerve Transplant

目的研究脱细胞异种面神经移植中环孢素A(cyclosporin A,CSA)的作用效果。方法 48只Wistar大鼠随机选择一侧作为实验侧,解剖面神经后于颊支制造1cm的神经缺损。按神经移植的种类分为4组,A组:异种面神经移植组;B组:异种面神经移植应用CSA免疫抑制组;C组:异种脱细胞神经移植组;D组:异种脱细胞神经移植应用CSA免疫抑制组。各组实验动物分别于术后5周,12周进行电生理学测试(潜伏期、运动神经传导速度),并进行光镜、电镜观察形态学变化。结果术后5周和12周时,C,D组动物在电生理指标及光镜、电镜指标上均优于A,B组,术后5周和12周,D组面神经颊支传导速度均高于其它各组。结论 1.0 cm缺损的异种脱细胞面神经移植动物实验中,应用CSA5mg/(kg.d)5周可以降低排斥反应,提高神经再生能力。

Objective To discuss the effect of cyclosporinA on acellular heterogeneous nerve transplant in Wistar rats.Methods 48 Wistar rats＇ each side were randomly expeimental side.After facial nerve were unveiled,1 cm defect were produced.All the animals were divided into 4 groups by kinds of graft nerve.Group A： heterogeneous nerve transplant;Group B： heterogeneous nerve transplant with CSA;Group C： acelluler heterogeneous nerve transplant;Group D： acelluler heterogeneous nerve transplant with CSA.5 and 12 weeks after operation,we carried through the electrophysiology（latent period,motor nerve conduction speed） and observed the morphological under light and trasmission electronic microscope.Results 5 weeks after operaton,the generation facial nerve of each group were incomplete and the function did not resume.12 weeks after operaton,the parameters of group C and D were better than group A and B.There were significant difference between group D and other groups on conduction velocity.Conclusion In the experiment of 1 cm facial nerve defect,the CSA can reduce immunoreaction and increase the facial regenarion.