纯镁及两种不同涂层纯镁的溶血现象

Hemolysis phenomenon of pure magnesium and two different coating pure magnesium

背景:尽管作为植入材料镁具有良好的生物相容性及可降解吸收等优点,但镁腐蚀过快,它的快速腐蚀将引起其机械性能在骨组织康复之前就显著下降。目的:检测纯镁及微弧氧化、二水磷酸氢钙涂层后的纯镁的血液相容性。方法:将纯镁及微弧氧化、二水磷酸氢钙两种涂层后的纯镁浸提液与抗凝稀释兔血接触,阴性对照组用为生理盐水,阳性对照为蒸馏水。混匀后离心,用分光光度计测定其上清液的A值,计算溶血率。结果与结论:未经处理的纯镁浸提液组溶血率为52.34%,微弧氧化涂层后的纯镁浸提液组溶血率为0.32%,二水磷酸氢钙涂层后的纯镁浸提液组溶血率为0.14%,阴性对照组溶血率为0,阳性对照组溶血率为100%。提示未经处理的纯镁出现了较严重的溶血现象,涂层后的纯镁血液相容性良好,溶血率小于5%,符合国家标准。

BACKGROUND：Although magnesium as implant material which has good biocompatibility and biodegradable absorption. However,excessive corrosion of magnesium will cause rapid decrease of the mechanical properties before rehabilitation of bone tissue. OBJECTIVE：To detect the blood-compatibility of pure magnesium（MG） ,micro-arc oxidized magnesium（MAO） and dicalcium phosphate dihydrate coating formed on micro-arc oxidized magnesium（DCPD） . METHODS：MG,MAO and DCPD extracts were contacted with anticoagulation dilution rabbit blood. Sodium Chloride was used in negative control group;distilled water was used in positive control group. Centrifuged after mixing,the optical density value of supernatant was determined by spectrophotometer,and hemolytic rate was counted. RESULTS AND CONCLUSION：Hemolytic rate of untreated pure magnesium extract group was 52.34%;the MAO and DCPDC extract group were 0.32% and 0.14%. Hemolytic rate of negative and positive group were 0% and 100%. The untreated pure magnesium showed a serious hemolysis. However,MAO and DCPD have excellent blood-compatibility,hemolytic rate is less than 5%,and they conform to the national standard requirement.