新型肝脏止血捆扎带的动物实验

Animal experimental research for new liver blood inflow banding

背景:课题组设计了一种尼龙搭扣肝止血捆扎带,比原有的止血带在技术上有创新,解决了临床使用中容易滑脱的问题,且使用更加简便。目的:观察新型肝脏止血捆扎带与全肝和半肝血流阻断法在肝切除中的效果比较。方法:健康家兔分别采用全肝血流阻断法切肝、半肝血流阻断法切肝及用新型肝脏止血捆扎带捆扎后切肝。比较术中肝脏缺血时间、手术时间、术中出血量,术前及术后第1,3,7天谷丙转氨酶、谷草转氨酶水平的变化。结果与结论:新型肝脏止血捆扎带组在手术时间和术中出血量上明显减少,且在术后第1天和术后第3天谷丙转氨酶和谷草转氨酶水平的升高幅度较其他两组低,但1周后均可恢复正常水平。结果提示该新型肝脏止血捆扎带术中操作简便,捆扎止血效果更为可靠,对肝功能损害较轻,是一种安全、有效、可行的新的肝切除方法。

BACKGROUND：A Velcro liver blood inflow banding was designed,with an innovation on the technology,which is easy to use and solve the clinical problem of easy to slip. OBJECTIVE：To compare the new liver blood inflow banding with vascular inflow occlusion（Pringle maneuver） and hemihepatic vascular occlusion in hepatectomy to provide suggestion for liver blood inflow occlusion. METHODS：The rabbits were divided randomly into 4 groups：control group,vascular inflow occlusion（Pringle maneuver） group,hemihepatic vascular occlusion group and new liver blood inflow banding group,each 10 rabbits. Liver blood inflow occlusion time,operative time,blood loss,alanine aminotransferase（ALT） and aspartate aminotransferase（AST） changes prior to and at 1,3,7 days after operation were observed and compared. RESULTS AND CONCLUSION：The new liver blood inflow banding group in operative time and blood loss was significantly reduced. While ALT and AST were significantly reduced 1 day and 3 days after surgery respectively,and came back to normal levels a week later. The new liver blood inflow banding not only blocks access to the liver blood flow,but also exempts from primary porta hepatis dissection,simplifies operation,makes it free from time restriction;it is a safe,effective and feasible new method of hepatectomy,and should be promoted in clinic.