应力环境组织培养法保存的人骨软骨移植物中胶原和基质金属蛋白酶组织抑制剂3

Collagen and tissue inhibitor of metalloproteinase-3 expression in human osteochondral allograft in storage media

背景:应力可以对体内外软骨组织或软骨细胞产生多种影响。目的:观察应力环境对普通组织培养基体外保存的骨软骨移植物保存质量的影响。方法:将正常人的骨软骨移植物分成3组:对照组即新鲜移植物,普通组移植物采用普通组织培养法保存,应力组用摇床作为动态加载装置对普通组织培养法保存的骨软骨移植物进行持续周期性应力加载。结果与结论:储存移植物2周时,Western blot法和透射电镜观察显示,与静态环境下保存相比,动态加载环境使关节软骨细胞外基质中基质金属蛋白酶组织抑制剂3的表达明显提高(P<0.01),能更好地保护基质中胶原成分的超微结构。结果证实,应力环境有利于保存人同种异体移植物软骨细胞和胶原的超微结构,动态非接触加载可提供一种更好的库存人同种异体骨软骨移植物的方法。

BACKGROUND： It has been well known that mechanical loading could affect articular cartilage;however,the effects of mechanical loading on articular cartilage stored in tissue media have rarely been reported so far.OBJECTIVE： To investigate the effects of loading environment on osteochondral allografts stored in tissue culture media.METHODS： Normal human osteochondral allografts were divided into three groups： control group,in which fresh grafts were used;common group,in which the osteochondral allografts were stored using common tissue culture media;mechanical loading group,in which osteochondral allografts stored in common tissue culture media were given a persistent periodical mechanical loading by a shaking table as a dynamic loading device.RESULTS AND CONCLUSION： At 2 weeks after storage,western blot analysis and transmission electron microscopy showed that the dynamic loading condition kept tissue inhibitor of metalloproteinase-3 expression and collagen fibril ultrastructure in articular cartilage significantly better than in static condition （P 0.01）.The results showed that the loading condition benefits the chondrocyte function and collagen ultrastructure in articular cartilage of human osteochondral allograft in storage media.The dynamic noncontact loading may provide a better method in storing human osteochondral allografts.