成骨细胞表面不同神经肽受体的表达

Expression of different neuropeptide receptors on osteoblast surface

背景:大量研究表明神经因素可调节骨代谢,迄今为止已发现5种神经肽参与骨代谢过程。目的:观察正常人成骨细胞表面不同神经肽受体的表达。方法:分别以降钙素基因相关肽、酪氨酸羟化酶、P物质、神经肽Y的单克隆抗体进行免疫组化染色,观察各种肽类受体在成骨细胞表面的表达情况。另外,利用计算机图像分析系统对染色灰度进行半定量分析。结果与结论:正常人成骨细胞表面有神经肽Y、P物质、酪氨酸羟化酶、降钙素基因相关肽受体表达,这些因子可以通过与相应的受体结合影响细胞生物学特性。不同因子免疫组化染色的灰度值由小到大依次为神经肽Y、降钙素基因相关肽、酪氨酸羟化酶、P物质,神经肽是成骨细胞活性的重要调节因子。

BACKGROUND：A large number of studies have demonstrated that neuropeptide can regulate bone metabolism.There have been five neuropeptides found to participate in the process of bone metabolism.OBJECTIVE：To observe the expression of different neuropeptides on the surface of osteoblasts of healthy adults.METHODS：Calcitonin gene-related peptide,tyrosine hydroxylase,P substance,neuropeptide Y monoclonal antibodies were used for immunohistochemical staining to observe the expression of various neuropeptide receptors on the surface of osteoblasts.In addition,semi-quantitative analysis of staining gray scale was performed through the use of computer image analysis system.RESULTS AND CONCLUSION：Neuropeptide Y,P substance and calcitonin gene-related peptide receptor expression was observed on the surface of osteoblasts of healthy adults and these factors influence cellular biological characteristics after binding with corresponding receptors.The gray value from low to high was in the following sequence,neuropeptide Y,calcitonin gene-related peptide,tyrosine hydroxylase,P substance.Results showed that neuropeptide is an important regulatory factor of biological activity of osteoblasts.