雌激素与葛根素组合治疗去卵巢大鼠骨症的最佳剂量

Effects of optimal dose of estradiol combined with puerarin on bone in ovariectomized rats

背景:小剂量的葛根素联合雌二醇对去卵巢大鼠骨质疏松症的治疗效果与单独使用较大剂量的葛根素或较大剂量的雌二醇效果相近。目的:寻找治疗Ⅰ型骨质疏松症最佳的雌二醇和葛根素的剂量搭配。方法:64只健康雌性大白鼠等分为假手术组、去卵巢模型组、葛根素-50组、雌二醇-200组、葛根素+雌二醇-5,50,100,150组。除假手术组外,其余大鼠均建立去卵巢动物模型。葛根素-50组大鼠皮下注射葛根素50mg/kg,1次/d;雌二醇-200组大鼠皮下注射雌二醇200μg/kg,2次/周;葛根素+雌二醇-5,50,100,150组大鼠皮下注射葛根素25mg/kg,1次/d同时分别注射雌二醇5,50,100,150μg/kg,2次/周。结果与结论:去卵巢模型组大鼠骨密度和骨钙、骨磷水平明显低于假手术组(P<0.05),骨组织呈骨质疏松的病理改变;葛根素和/或雌二醇治疗10,20周后骨密度和骨钙、骨磷水平明显升高(P<0.05),其中葛根素+雌二醇-100组治疗去卵巢大鼠骨质疏松的效果最为明显,提示葛根素25mg/kg,1次/d+雌二醇100μg/kg,2次/周是治疗去卵巢大鼠骨质疏松的最佳搭配剂量。

BACKGROUND：Low dose of puerarin combined with estradiol shows similar effects to high dose of puerarin or estradiol on treatment of osteoporosis in rats.OBJECTIVE：To search the optimal doses for estradiol and puerarin for treatment of typeⅠosteoporosis.METHODS：Sixty-four healthy female rats were randomly divided into eight experimental groups（n = 8）：sham-operated,ovariectomized,puerarin-50,estradiol-200,puerarin＋estradiol-5,50,10,150.All rats were subjected to ovariectomy except those in the sham-operated group.Rats in the puerarin-50 group were subcutaneously injected with 50 mk/kg puerarin,once a day.Rats in the estradiol-200 group were subcutaneously injected with 200 μg/kg estradiol,twice a week.Rats in the puerarin＋ estradiol-5,50,10,150 groups were subcutaneously injected with 25 mg/kg puerarin,once a day and simultaneously injected with 5,50,10,150 μg/kg estradiol,twice a week.RESULTS AND CONCLUSION：Compared with sham-operated group,bone mineral density,bone calcium,and bone phosphorous of rats were significantly lower in the ovariectomized group（P 0.05）,and bone tissue presented with the pathological change of osteoporosis.After treatment with puerarin and/or estradiol for 10 and 20 weeks,bone mineral density,bone calcium,and bone phosphorous levels were significantly increased（P 0.05）,and puerarin＋estradiol-100 group acquired the most obvious curative effects on osteoporosis.The results showed that 25 mg/kg puerarin（once a day）and 100 μg/kg estradiol（twice a day）is the best dose for treatment of osteoporosis in ovariectomized rats.