雌二醇对铅中毒模型大鼠骨组织及骨代谢指标不能产生影响

Estradiol does not affect bone tissue and bone metabolism indices in rat models of lead poisoning

背景:关于铅中毒能否引起骨质疏松症及雌激素对其治疗是否有效尚无共识。目的:观察雌二醇对去卵巢大鼠和铅中毒大鼠所致骨质疏松症的治疗效果。方法:雌性大白鼠100只等分成正常对照组、去卵巢模型组、染铅模型组、雌二醇+去卵巢组和雌二醇+染铅模型组。建模后1周雌二醇+去卵巢组和雌二醇+染铅模型组皮下注射雌二醇(100μg/kg),2次/1周,连续12周。结果与结论:在去卵巢模型组和铅中毒组中骨钙、骨磷、血钙及血磷均出现降低(P<0.01),血碱性磷酸酶升高(P<0.01),骨组织形态呈现骨质疏松的病理改变。雌二醇+去卵巢组的骨代谢生化指标血钙、磷、和碱性磷酸酶和骨组织形态均恢复正常,而雌二醇+铅中毒组的骨代谢生化指标和骨组织形态呈现骨质疏松未出现明显改善迹象。铅中毒组和雌二醇+铅中毒组的骨铅和血铅明显高于正常对照组(P<0.01)。说明铅中毒可引起骨质疏松的病理改变,雌二醇对去卵巢大鼠的骨质疏松症有良好治疗效果,而对铅中毒所致的骨质疏松症无明显疗效。

BACKGROUND：There has been no consensus regarding whether lead poisoning can cause osteoporosis and whether estradiol exhibits curative effects on osteoporosis.OBJECTIVE：To observe the curative effects of estradiol on osteoporosis-induced by ovariectomy and lead poisoning in rats.METHODS：A total of 100 female rats were randomly and evenly divided into five groups：normal control,ovariectomized,lead poisoning,estradiol＋ovariectomized and estradiol＋lead poisoning.At 1 week after ovariectomy,estradiol（100 μg/kg）was subcutaneously injected into the rats in the estradiol＋ovariectomized and estradiol＋lead poisoning groups,twice a week,for successive 12 weeks.RESULTS AND CONCLUSION：In the ovariectomized group and lead poisoning group,calcium and phosphorus levels in the bone and serum were significantly decreased（P 0.01）,serum level of alkaline phosphatase was significantly increased（P 0.01）,and bone tissue showed the pathological change of osteoporosis.In the estradiol＋ovariectomized group,serum levels of calcium,phosphorus,and alkaline phosphatase and bone tissue morphology returned to normal,while in the estradiol＋lead poisoning group,no obvious changes in bone metabolism indices and bone tissue morphology above-mentioned did not recover obviously.The lead level in the bone and serum was significantly higher in the lead poisoning group,estradiol＋lead poisoning group than in the normal control group（P 0.01）.These results showed that lead poisoning can cause osteoporosis,and estradiol exhibits good curative effects on osteoporosis-caused by ovariectomy in rats,but it does not produce obvious curative effects on osteoporosis-caused by lead poisoning.