改构型酸性成纤维细胞生长因子对衰老模型大鼠自由基与脂质过氧化物的影响

Effects of modified acidic fibroblast growth factor on free radical and lipid peroxide in aging model rats

背景:有研究表明改构型酸性成纤维细胞生长因子是多功能生长因子,但其抗衰老作用至今尚未见报道。目的:观察改构型酸性成纤维细胞生长因子对D-半乳糖致衰老大鼠脑组织、肝组织及血清中超氧化物歧化酶活力、丙二醛含量和抑制羟自由基能力的影响。方法:选择成年Wistar大鼠皮下注射D-半乳糖建立衰老模型,建模成功后随机分为模型组、生理盐水对照组和改构型酸性成纤维细胞生长因子治疗组,另设正常对照组。改构型酸性成纤维细胞生长因子组按12μg/kg剂量肌肉注射改构型酸性成纤维细胞生长因子,生理盐水对照组肌肉注射等量的生理盐水,模型组不作干预。结果与结论:与模型组和生理盐水对照组相比,改构型酸性成纤维细胞生长因子治疗组脑组织、肝组织及血清中超氧化物歧化酶活力和抑制羟自由基能力均显著升高(P<0.01或P<0.05),丙二醛含量均显著降低(P<0.01或P<0.05)。结果证实,改构型酸性成纤维细胞生长因子可通过降低自由基,提高机体的抗氧化能力来发挥延缓衰老的作用。

BACKGROUND：Several studies have demonstrated that modified acidic fibroblast growth factor（maFGF）is a multifunctional growth factor,but its anti-aging effects have not been reported.OBJECTIVE：To investigate the effects of maFGF on superoxide dismutase（SOD）,malondialdehyde（MDA）and hydroxyl radical ability in cerebral cortex,liver tissue and serum of subacute aging model rats.METHODS：Adult Wistar rats were established into aging models by subcutaneous injection of D-galactose.Then aging rat models were assigned to three groups：model,saline control,maFGF.A normal control group was set.The maFGF group rats were intramuscularly injected with 12 μg/kg maFGF.The saline control group was intramuscularly injected with the same amount of saline.The model group rats did not receive any intervention.RESULTS AND CONCLUSION：Compared with model and saline control groups,SOD activity and hydroxyl radical ability in cerebral cortex,liver tissue and serum were significantly increased（P 0.01 or P 0.05）,and MDA level was significantly decreased（P 0.01 or P 0.05）in the maFGF group.Results showed that maFGF decreases free radial level,enhances organism＇s anti-aging ability,and thereby exhibiting anti-aging effects.