紫外激光辐射区肝组织β连环蛋白及过氧化物酶体增殖物激活受体γ蛋白的表达

Beta-catenin and peroxidase proliferator-activated receptor gamma expressions in hepatic tissue treated by 355 nm laser ablation

背景:研究表明经典的wnt信号途径可通过抑制成脂分化关键因子过氧化物酶体增殖物激活受体γ而抑制细胞向脂肪细胞的分化。目的:观察紫外光辐射肝组织致创后自然恢复期不同阶段β连环蛋白及过氧化物酶体增殖物激活受体γ的表达。方法:将雄性SD大鼠随机分4组:激光致创组大鼠是以355nm紫外激光辐射肝叶形成损伤区,器械致创对照组则以手术剪肝叶致创,假手术组仅做腹部切开及缝合,正常组大鼠仅实施正常喂养。结果与结论:紫外激光可在肝组织形成边缘整齐的消融区并在周围形成可自然修复的有限致损区域。免疫组织化学图像平面测量表明紫外激光辐射后自然恢复1周大鼠肝组织中β连环蛋白表达低于各对照组(P<0.01),而自然恢复表达β连环蛋白辐射区周围组织表达高于正常(P<0.01)。蛋白印迹结果显示辐射致创组辐射后自然同时过氧化物酶体增殖物激活受体γ蛋白2周恢复高于1周恢复(P<0.01),且恢复期肝组织中可同时表达过氧化物酶体增殖物激活受体γ1及2。结果证实,辐射区旁组织自然恢复各阶段存在不同的β连环蛋白及过氧化物酶体增殖物激活受体γ表达调控,2周自然恢复后辐射区旁组织成脂进程得到加强。

BACKGROUND：Several studies have demonstrated that wnt signaling pathway inhibits adipogenic differentiation through inhibiting the expression of peroxisome proliferator-activated receptor gamma（PPARγ）,a key factor of adipogenic differentiation in hepatic tissue.OBJECTIVE：To determine the expressions of β-catenin and PPARγ in hepatic tissue treated by 355 nm laser ablation at different stages of natural recovery stage.METHODS：Male SD rats were randomized divided to four groups.In the ultraviolet beam ablation group,355 nm ultraviolet beam was used on hepatic tissue targeted zone to obtain an ablation damage area.In the mechanical damage group,mechanical damage was created through the use of operating scissors.In the sham operation group,abdominal incision and suture were performed,without other procedures.In the normal control group,rats were routinely raised without any procedures.RESULTS AND CONCLUSION：Hepatic tissue obtained a regular ablation damage zone and a recoverable para-damage area by ultraviolet laser beam radiation.Immunohistochemistry results showed that β-catenin expression was significantly lower after 1-week natural recovery in the ultraviolet beam ablation group compared with other groups（P 0.01）,but its expression was significantly higher in the para-damage area after 2-week recovery compared with the normal control group（P 0.01）.Western blot assay showed that PPARγ expression in the ultraviolet beam ablation group was significantly increased after 2-week natural recovery than 1-week natural recovery（P 0.01）,and PPARγ had a co-expression with its iosform 1 and 2 during the natural recovery stage.The results suggest that β-catenin and PPARγ expression pattern tightly depended on nature recovery duration,and adipogenic process in the para-damage area in the hepatic tissue was strengthened after 2-week natural recovery.