胶原诱导性关节炎大鼠滑膜组织中磷酸化p38与JNK通路及痹肿消汤的影响

Effects of Bizhongxiao decoction on activation of phosphorylated p38 and JNK proteins in synovium of rats with collagen-induced arthritis

背景:有研究报道滑膜细胞信号传导异常是类风湿关节炎发病的重要机制之一。目的:观察胶原诱导性关节炎大鼠中丝裂原活化蛋白激酶通路中磷酸化p38和磷酸化JNK的活化及痹肿消汤的影响。方法:将72只SD大鼠随机分为正常组、模型组、痹肿消汤组。模型组及痹肿消汤组大鼠足趾注射胶原蛋白乳剂制备胶原诱导性关节炎模型大鼠,10d后加强免疫,初次免疫后第14天开始给痹肿消汤组大鼠每天痹肿消汤灌胃,模型组蒸馏水灌胃,正常组自由饮水。结果与结论:Westernblot检测结果显示,胶原诱导性关节炎大鼠中磷酸化p38及JNK的表达较正常组显著增高(P<0.05)。与模型组相比,痹肿消汤组能下调磷酸化p38及JNK蛋白的表达(P<0.05)。说明痹肿消汤可抑制类风湿关节炎中磷酸化p38及JNK的高表达。

BACKGROUND：Reports have shown that abnormal synovial cell signaling is one of important mechanisms of rheumatoid arthritis.OBJECTIVE：To investigate the effects of Bizhongxiao decoction on activation of phosphorylated p38 and JNK proteins in mitogen-activated protein kinase（MAPK）pathway in synovium of rats with collagen-induced arthritis.METHODS：A total of 72 Sprague-Dawley rats were randomly and evenly divided into three groups：normal,model,and Bizhongxiao decoction.In the model and Bizhongxiao decoction groups,rat models of collagen-induced arthritis were created by injection of typeⅡcollagen and complete Freud＇s adjuvant into rat toes.After 10 days,rats were immunized again.From day 14 after primary immunization,the Bixiaozhong decoction group rats were intragastrically administered Bizhongxiao decoction every day,the model group rats were identically given distilled water,and the normal group rats were allowed to drink water freely.RESULTS AND CONCLUSION：Western blot analysis results showed that phosphorylated p38 and JNK expressions were significantly greater in the collagen-induced arthritis rats than in the normal controls（P 0.05）.Compared with the model group,phosphorylated p38 and JNK expressions were significantly downregulated in the Bizhongxiao decoction group（P 0.05）.These findings suggest that Bizhongxiao decoction can inhibit phosphorylated p38 and JNK expressions in rheumatoid arthritis.