摘要
背景:体内体外实验研究证明,静磁场具有促进成骨细胞分化及骨形成的作用。目的:观察静磁场作用下金属离子对成骨细胞毒性的影响。方法:将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液,将金属离子Co2+、Cr3+分别与小鼠颅骨成骨细胞(MC3T3-E1)在不同的磁场强度(1,10,100mT)下共培养。实验分4组:设Co2++Cr3+组(对照组)和Co2++Cr3+分别+1,10,100mT组。结果与结论:恒磁场作用下的成骨细胞呈现更加成熟的形态学特征,Co2+、Cr3+金属离子对成骨细胞MC3T3的毒性明显降低;同时G2M(分裂期)分布比例明显增加,细胞停滞在G0G1(休眠期)的比率明显减少;与对照组相比,不同场强磁场加载后,成骨细胞碱性磷酸酶活性明显增强(P<0.05)。提示一定强度的静磁场可拮抗Co2+、Cr3+金属离子对成骨细胞的毒性作用。
BACKGROUND:Static magnetic field can promote the proliferation,differentiation and functional activity of osteoblasts.OBJECTIVE:To investigate the toxic effect of metal ions Co2+,Cr3+ on osteoblasts under the static magnetic field.METHODS:CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water.Mouse calvarial osteoblasts(MC3T3-E1) were cultured with Co2+ and Cr3+ ions in different magnetic field of 1,10,100 mT.Cells only cultured with Co2+ and Cr3+ ions were taken as control group.RESULTS AND CONCLUSION:Under the static magnetic field,osteoblasts showed more mature morphological features;the toxic effect of metal ions Co2+ and Cr3+ on osteoblasts reduced significantly;G2M(dividing phase) distribution ratio increased and G0G1(dormancy stage) distribution ratio decreased;the A value tested by alkaline phosphatase activity in the three experiment groups up-regulated obviously.The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co2+ and Cr3+ on osteoblasts.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第35期6567-6570,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30760255)
南昌大学学科交叉基金(Z03722)~~