静磁场作用下金属离子对成骨细胞的毒性

Static magnetic field reduces the toxic effect of metal ions on osteoblasts

背景:体内体外实验研究证明,静磁场具有促进成骨细胞分化及骨形成的作用。目的:观察静磁场作用下金属离子对成骨细胞毒性的影响。方法:将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液,将金属离子Co2+、Cr3+分别与小鼠颅骨成骨细胞(MC3T3-E1)在不同的磁场强度(1,10,100mT)下共培养。实验分4组:设Co2++Cr3+组(对照组)和Co2++Cr3+分别+1,10,100mT组。结果与结论:恒磁场作用下的成骨细胞呈现更加成熟的形态学特征,Co2+、Cr3+金属离子对成骨细胞MC3T3的毒性明显降低;同时G2M(分裂期)分布比例明显增加,细胞停滞在G0G1(休眠期)的比率明显减少;与对照组相比,不同场强磁场加载后,成骨细胞碱性磷酸酶活性明显增强(P<0.05)。提示一定强度的静磁场可拮抗Co2+、Cr3+金属离子对成骨细胞的毒性作用。

BACKGROUND：Static magnetic field can promote the proliferation,differentiation and functional activity of osteoblasts.OBJECTIVE：To investigate the toxic effect of metal ions Co2＋,Cr3＋ on osteoblasts under the static magnetic field.METHODS：CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water.Mouse calvarial osteoblasts（MC3T3-E1） were cultured with Co2＋ and Cr3＋ ions in different magnetic field of 1,10,100 mT.Cells only cultured with Co2＋ and Cr3＋ ions were taken as control group.RESULTS AND CONCLUSION：Under the static magnetic field,osteoblasts showed more mature morphological features;the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblasts reduced significantly;G2M（dividing phase） distribution ratio increased and G0G1（dormancy stage） distribution ratio decreased;the A value tested by alkaline phosphatase activity in the three experiment groups up-regulated obviously.The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblasts.