东部沿海贝类中副溶血弧菌的分布及特征

Distribution and Characterization of Vibrio parahaemolyticus in Shellfish from Eastern Coast of China

为调查我国东部沿海城市零售贝类中副溶血弧菌的分布情况及其血清型和分子特征。共采集了288个贝类样品,采用MPN-PCR方法进行定量研究,分离得到的菌株用RAPD方法进行分子分型并考察其毒力基因携带情况。结果显示,贝类中副溶血弧菌含量季节分布明显,夏季显著高于其他季节(P<0.05);共分离得到172株副溶血弧菌,其中157株可区分其O群,42株可区分其K型,O3群菌株最多,占19.1%;RAPD结果显示,172株菌共有73种带型,可分成18个类群;共检测到2株菌含tdh基因,5株菌含trh基因,未检测到同时含上述两种基因的菌株;2株含tdh基因菌株的血清型分别为O3:K6和O4:K68,RAPD结果显示它们属于同一个类群。

To investigate the distribution,serological and molecular characterization of vibrio parahaemolyticus of shellfish,a total of 288 samples were collected and analyzed in this manuscript.The levels of V.parahaemolyticus were estimated by MPN-PCR and isolated strains were clustered by RAPD analysis.The tdh and trh genes were detected by PCR.The results showed that the distribution of V.parahaemolyticus was disparity in season,the level of V.parahaemolyticus in summer were different from other seasons（P〈0.05）.Altogether 172 strains were isolated and identified,of which 157 strains were typed by O-grouping serum and 42 strains were typed by K-typing serum,the group of O3（19.1%） was the most frequently involved in shellfish.RAPD analysis showed that there were 73 different patterns which were clustered into 18 groups.Two isolates showed positive of tdh gene and 5 isolates showed positive of trh gene,there was no strain with tdh and trh genes simultaneously.The serovars of two isolates with tdh gene were O3∶K6 and O4∶K68 and two isolates were grouped into the same cluster by RAPD analysis.