猪三毛滴虫对4种药物的体外药敏试验

In vitro susceptibility of Tritrichomonas suis to four antimicrobial agents

目的探讨硝唑尼特、替硝唑、甲硝唑和制霉菌素4种药物对猪三毛滴虫的作用效果,以期为临床用药提供依据。方法在体外培养条件下,用24h敏感性试验确定4种药物对猪三毛滴虫24h的最低致死浓度(MLC);绘制时间-杀虫曲线,分析不同浓度受试药物作用不同时间对猪三毛滴虫的杀灭效果。结果 4种药物均具有不同程度的抑制或杀灭猪三毛滴虫的作用,其中硝唑尼特杀灭猪三毛滴虫的效果较好,24hMLC为12.50mg/L;替硝唑和甲硝唑24hMLC均为25.00mg/L,但时间-杀虫曲线显示替硝唑效果好于甲硝唑;制霉菌素的杀虫效果较差。结论猪三毛滴虫对硝唑尼特和替硝唑敏感。

Objective To study the effects of tinidazole,metronidazole,nitazoxanide,and nystatin on Tritrichomonas suis in order to provide a basis for clinical treatment of the disease.Methods T.suis was subjected to a 24-hour susceptibility assay in vitro with four agents to determine the twenty-four-hour minimum lethal concentration of each agent.Time-kill curves of the different concentrations of drugs tested were used to clarify the effectiveness with which T.suis was killed at different times.Results All four agents inhibited or killed T.suis to a different degree.Nitazoxanide was most effective,with a 24-hour MLC of 12.50 mg/L.The 24-hour MLC for metronidazole and tinidazole was 25.00 mg/L,but tinidazole had a better time-killing curve than did metronidazole.Nystatin was the least effective.Conclusion T.suis is sensitive to tinidazole and nitazoxanide.