摘要
从农田土壤及池塘水中分离得到共2株降解内分泌干扰物辛基酚聚氧乙烯醚(OPEOn)的细菌X-4及X-6.经过对其形态特征、生理生化以及16S rDNA序列分析,菌株X-4初步鉴定为球形芽孢杆菌(Bacillus sphaeri-cus),菌株X-6初步鉴定为嗜水气单胞菌(Aeromonas hydrophila)或点状产气单胞菌点状亚种(Aeromonas puncta-ta)。通过摇瓶实验考察了生长条件对OPEOn降解的影响,得出X-4及X-6降解OPEOn的最佳条件是接种量为1.0%,初始pH值为7.3～7.5,温度为30℃。在该条件下,对菌株X-4及X-6在不同初始浓度OPEOn下的降解反应过程进行动力学分析,该降解反应在低质量浓度(5,20,50,100mg/L)时符合一级反应动力学特征,而在较高底物质量浓度(200mg/L,1000mg/L)下不能用一级反应动力学描述,且2株菌对OPEOn的降解率在初始浓度为20mg/L时达到最大值,分别为X-4:26%,X-6:32%。
Two strains of octylphenol polyethoxylate（OPEOn）degrading, X-4 and X-6 were separated and obtained from farmland soil（X-4）and pond water（X-6）. The strains were identified preliminarily as Bacillus sphaericus for X-4, Aeromonas hydrophila or Aeromonas punctata for X-6 based on analysis in terms of morphological characteristics, physiological and biochemical characterization, as well as sequence analysis of 16S rDNA. Growth environment for the two strains were tested though a designed shaking flasks experiment, The optimum condition indicate that pH remain at 7.3-7.5, ,temperature reach 30 ℃, with inoculum size 1.0 % for X-4 and 1.5 % for X-6, respectively. Under such circumstances, the degradation reaction are consistent with one order kinetic characteristic in the case of lower initial level for strains（5,20,50,100 mg/ L）, while it failed to follow one order kinetic characteristic, while higher initial sample level present（200 mg/L, 1 000 mg/L）. Furthermore, when the initial concentration for strains registered 20mg/ L, the degradation rate maximized with 26% for X-4 and 32% for X-6, respectively.
出处
《三峡环境与生态》
2011年第5期9-13,共5页
Environment and Ecology in the Three Gorges
