IL-1B+3954C/T和IL-1B-511C/T基因多态性和种植体周围炎的相关性研究

Relationship between implantitis and polymorphisms of the interleukin-1B(+3954C/T,-511C/T)gene

目的:探讨研究种白介素1(IL-1)基因+3954和-511位点单核酸多态性和种植体周围炎的关系以及临床意义。方法:采用病例对照实验设计,选取种植体炎患者以及对照组各50名,应用聚合酶链反应—限制性内切酶片段长度多态性基因分析方法研究两组IL-1B基因+3954位点和-511位点基因型和等位基因分布特点,并探讨其与种植体周围炎的相关性。结果:IL-1B基因+3954位点CC、CT基因型检出率为93%和7%,无TT基因型,两种基因型和C、T等位基因频率在组间分布差异无统计学意义(P>0.05);IL-1B基因-511位点主要以CT为主,检出率为58%,CC、CT基因型检出率都为21%。结论:排除吸烟,牙周炎病史等影响因素后,IL-1B基因+3954位点和-511位点的多态性与种植体周围炎炎的易感性无关。

Objective：The purpose of the study was to investigate the association of chronic periodontitis with interleukin-1B（＋3954C/T,-511C/T） gene polymorphisms.Methods：The genotype and allele distributions of interleukin-1B in a case-control study involving 50 patients with implantitis and 50 health controls were analyzed by polymerase chain reaction-restriction fragment length polymorphism.Results：The frequencies of CC,CT,TT genotype at the site ＋3954 were 93%,7%,0% and the frequencies of CC,CT,TT genotype at the site-511 were 21%,58%,21%.So there were not any significant differences in genotype or allele frequencies between groups for interleukin-1B（＋3954C/T,-511C/T）gene polymorphisms.Conclusion：excluded some influence factors,such as smoking and periodontitis,there was no relationship between and interleukin-1B（＋3954C/T,-511C/T）polymorphisms.