宣威肺腺癌xwlc-05和肺腺癌A549细胞株体外培养细胞活力的比较研究

The Comparison of Cell Viability between Xuanwei Lung Adenocarcinoma Cell Lines xwlc-05 and Cell Lines A549 in Vitro

目的比较宣威肺腺癌xwlc-05和肺腺癌A549细胞株体外培养细胞活力.方法体外培养宣威肺腺癌xwlc-05和肺腺癌A549细胞株,观察细胞形态变化.取传代培养后1 d、3 d、5 d 3个时间点分别计数细胞,MTT检测3时间点细胞活力变化,并进行统计学分析比较.结果宣威肺腺癌xwlc-05细胞株和肺腺癌A549细胞株各时间点比较,培养1 d、3 d、5 d组的细胞数量逐渐增加,差异有统计学意义(P<0.05);同时,宣威肺腺癌xwlc-05细胞株和肺腺癌A549细胞株3 d组细胞活力较1 d组增加,而5 d组的细胞活力较3 d组下降,差异有统计学意义(P<0.05).结论不同肺腺癌细胞株体外增殖和细胞活力不完全一致.

Objective To compare cell viability between Xuanwei lung adenocarcinoma cell lines xwlc-05 and cell lines A549 in vitro.Methods Xuanwei Lung adenocarcinoma cell lines xwlc-05 and cell lines A549 were cultured and morphological changes were observed.The number of adenocarcinoma cells and the cell viability were tested and statistically analyzed at 3 time points after culturing for 1 day,3 days and 5 days.Results The number of lung adenocarcinoma cell lines xwlc-05 and A549 gradually increased on 1 day,3 days,5 days after culture.At the same time,the cell viability of xwlc-05 gradually increased,while it exhibited statistical significant decrease on A549（P0.05）.Conclusion Different lung adenocarcinoma cell lines have different cell viability and proliferation ability in vitro.