孕期脂多糖刺激对子代大鼠血管肾素血管紧张素系统的影响

Prenatal exposure to lipopolysaccharide results in vascular renin-angiotensin system dysfunction in offspring rats

目的探讨孕期炎症刺激对子代大鼠血管局部肾素血管紧张素系统的影响。方法SpragueDawley（SD）孕鼠6只随机分为对照组和脂多糖（LPS）组，于孕期第8、10、12天分别给予生理盐水和LPS0．79mg／kg（腹腔注射），子代大鼠出生后采用无创伤尾动脉方法测定血压，免疫组织化学方法测定胸主动脉血管紧张素Ⅱ（AngⅡ）蛋白的表达水平，Westernblot方法测定血管局部血管紧张素Ⅱ型受体（AT，R）、血管紧张素112型受体（AT2R）的蛋白含量。结果LPS组子代大鼠出生后第12周血压明显高于对照组子代大鼠（P〈0．01），15周龄胸主动脉血管组织的AngⅡ表达明显高于对照组子代大鼠（P〈0．01），AT1R蛋白表达明显高于对照组子代大鼠（P〈0．05），AR2R蛋白表达低于对照组子代大鼠，但差异无统计学意义，AT1R／AT2R比值明显高于对照组子代大鼠（P〈0．01）。结论孕期炎症刺激导致子代大鼠血管局部肾素血管紧张素系统异常，可能是子代大鼠高血压发生发展的重要原因。

Objective To explore the impact of prenatal exposure to lipopolysaccharide on renin- angiotensin system of offspring rats. Methods Six pregnant SD rats were randomly divided into 2 groups. The rats in the lipopolysaccharide （LPS） group were treated with LPS 0. 79 mg/kg （i. p. ） on the 8th, 10th and 12th day of gestation, and rats in the control group were treated with saline at the same time points. The blood pressure of offspring rats was measured by the tail cuff method. Protein expression of Angiotensin Ⅱ （AngⅡ ） in thoracic aorta vessel was determined by immunohistoehemisry. Protein expressions of Ang Ⅱ type 1 and type 2 receptor in thoracic aorta vessel were detected by Western blot. Results Blood pressure of 12-week-old offspring rats of LPS group was significantly higher than that of 12-week-old offspring rats of control group（P 〈 0. 01 ）. The protein expression of Ang Ⅱ and Ang Ⅱtype 1 receptor in thoracic aorta vessel were significantly higher while protein expression of Ang Ⅱ type 2 receptor was lower in 15-week-old offspring rats of LPS group than in control group, resulting in a significant increase in the ratio of Ang Ⅱ type 1 reeeptor/AngⅡ type 2 receptor in the aorta at 15-week-old of offspring rats than in 15-week-old offspring rats of control group（P 〈0. 01 ）. Conclusion Prenatal lipopolysaecharide exposure results vascular renin-angiotensin system dysfunction, which may play an important role on the pathogenesis of hypertension development in offspring rats.