新疆维吾尔族和汉族人群Tim-3基因多态性与变应性鼻炎的相关性研究

Relationship between polymorphism sites of Tim-3 and allergic rhinitis in a population of Uigurs and Hans from Xinjiang Uigur Autonomous Region of China

目的研究探讨新疆地区维吾尔族（维族）、汉族人群Tim-3基因5′侧翼区的rs10515746（-574G〉T）、rs4704853（-882C〉T）及位于内含子区的rs13170556和rs6555849位点的单核苷酸多态性（single nueleotide polymorphism，SNP）及其与变应性鼻炎（allergic rhinitis，AR）的关系。方法采用SNaPshot SNP分型技术，对新疆地区维族和汉族AR患者及健康对照人群的Tim-3基因rs10515746（-574G〉T）、rs4704853（-882C〉T）、rs13170556和rs6555849位点的SNP进行分析，根据Tim-3基因分布计算其基因型和等位基因频率，并采用Hardy—Weinberg平衡定律进行检验。结果维族人群中rs10515746（-574G〉T）、rs4704853（-882C〉T）、rs13170556和rs6555849位点基因型频率及其等位基因频率在AR组与对照组间差异均无统计学意义（P值均〉0．05）；汉族人群中基因型频率及其等位基因频率在AR组与对照组之间差异也无统计学意义（P值均〉0．05）。除汉族病例组rs4704853位点外，其他各位点基因型及等位基因频率分布符合Hardy—Weinberg平衡定律。结论新疆地区维族、汉族人群中Tim-3基因rs10515746（-574G〉T）、rs4704853（-882C〉T）、rs13170556和rs6555849位点存在多态性变异，但与AR的易感性无关。

Objective To find the relationship between the polymorphism of the Tim-3 gene mononucleotide site rs10515746 （ - 574G 〉 T）, rs4704853 （ - 882C 〉 T）, rs13170556, rs6555849 and the allergic rhinitis （AR） susceptibility in a population of Uigurs and Hans from Xinjiang Uigur Autonomous Region of China. Methods The two mononucleotide polymorphism site of Uigur and HaM AR patients and normal people were analyzed in Xinjiang in terms of SNaPshot SNP typing technique. The gene type and alleomorph frequency were than caculated. According to the Tim-3 gene layout, the gene type and the allelomorph frequency had been calculated. The Hardy-Weinberg balance law had been used for exam ination. Results There were no statistic differences between the genotype and alleles of each frequency [ site rs10515746（ -574G 〉 T） , rs4704853 （ -882C 〉 T） , rs13170556 and rs6555849） ] of Uighurs, in group AR and matched group （ all P 〉 0.05 ）. There were no statistic differences between the genotype and alleles of each frequency of Han people in group AR and matched group （ all P 〉 0.05 ）. Except Han eases site rs4704853, the distribution of other genotype and alleles of each frequency accord with heredity balance through the Hardy-Weinberg balance law. Conclusions In Xinjiang Uigur autonomous region, locus of gene Tim-3, site rs10515746（-574G 〉T）, rs4704853 （-882C 〉T）, rs13170556 and rs6555849, has the polymorphism of the Tim-3 gene mononucleotide. There is no correlation between AR susceptibility and Tim- 3 gene mononucleotide site of Uigur people and HaM people in Xinjiang.