人牙周膜成纤维细胞在微弧氧化陶瓷膜表面的生物相容性研究

Experiment of the microarc oxidation film′s biocompatibility to human periodontal ligment fibroblast

目的观察通过微弧氧化(MAO)和水热处理形成的陶瓷膜表面人牙周膜成纤维细胞(hPDLF细胞)的粘附和增殖情况,评价微弧氧化陶瓷膜表面的生物相容性及MAO工艺应用于钛植入材料表面处理的可行性。方法纯钛试件分为3组,微弧氧化处理组(M组)、微弧氧化-水热处理组(M-H组)和纯钛对照组,每组各20枚。对不同时间点hPDLF细胞在材料表面的附着、生长、增殖情况以及ALP活性进行检测,并对实验结果进行统计分析。结果陶瓷膜具有良好的生物相容性,培养5 d后,改性两组材料的表面细胞增殖比纯钛组及空白对照组明显增多,各组间细胞增殖情况存在统计学差异。同时培养4d后,M组和M-H组细胞ALP活性大幅提高,各组间比较均有统计学差异。结论 MAO处理后的纯钛表面对hPDLF的生物相容性优于未处理组,增加水热处理工艺较一般MAO工艺可以更有效提高hPDLF细胞的早期粘附、增殖及ALP活性。

Objective To study the biocompatibility of human periodontal ligment fibroblast（hPDLF）on pure titanium surface treated with MAO and hydrothermal treatment.Methods 60 samples of pure titanium were prepared and separated into three groups：M group with MAO treatment,M-H group with MAO and hydrothermal treatment,Ti group without treatment.Attachment,proliferation and ALP activity of hPDLF cultured on the titanium surfaces were assessed at different time.All data were statistically analysed.Results The MAO film showed its excellent biocompatibility to hPDLF.Cell attachment on the surfaces of M group and M-H group were higher than that on surfaces of Ti group.hPDLF proliferation and ALP activity were higher on surfaces of M-H goup than those on surfaces of the other two groups（P0.05）.Conclusions Titanium surface treated with MAO exhibits better biocompatibility to hPDLF than surfaces of pure titanium,and hydrothermal treatment may increase original cell attachment,proliferation and ALP activity.