猕猴肝脏实验感染猪囊尾蚴的观察

Observation on experimental macaque infected with cysticercus

目的了解猪囊虫病猕猴的肝功能、肝脏声像图及病理变化特征。方法用猪带绦虫虫卵经喂食感染健康猕猴,于感染第84d进行猕猴肝脏B超检查和血清部分肝功能指标的全自动生化分析仪检测、透明质酸的放射免疫法检测以及猪囊尾蚴IgG的ELISA检测。于感染第90d,经麻醉致猕猴死亡,剖检各组织器官,取肝脏进行常规组织病理学检查。结果猕猴经人工感染猪带绦虫幼虫84d后,体重较感染前减轻2.4kg,肝脏B超检查见边界较清楚、不均匀的低回声或混合性回声的结节样病灶;血清总胆汁酸盐为感染前的4.5倍,透明质酸为感染前的1.9倍,胆碱酯酶较感染前降低25%;肝组织活检见11个结节,结节内未见虫体,但病灶内可见嗜酸性粒细胞浸润,周围纤维结缔组织增生,可见肝细胞脂肪变性和坏死;血清猪囊尾蚴IgG阳性。结论猪囊尾蚴除可引起灵长类的肌肉和脑囊虫病,还可寄生在肝脏并引起寄生部位肝组织的损伤,血清学检查和肝脏B超有助于诊断肝脏囊虫病。

The aim of this study was to understand liver function,ultrasonography and pathological changes in liver of the macaque with cysticercosis.A macaque was infected with eggs of T.solium through digestive tract.Color Doppler ultrasound examination,automatic biochemical analysis of some indicators for liver function,radioimmunoassay of hyaluronic acid and ELISA of anti-cysticercus IgG in sera were carried on the macaque which had been infected for 84 days.Euthanasia was done to the macaque by injection of excessive anesthetic at 90 days after infection.Then distribution and development of the larvae in macaques＇ tissue and pathological changes were observed.Results showed that the macaque lost weight by 2.4 kg after being infected by T.solium larvae for 84 days,and several nodules on the liver could be found by color Doppler ultrasound examination.Concentration of hyaluronic acid and total bile acid were higher than that of the pre-infection sample,whereas the concentration of choline esterase was lower.ELISA of anti-cysticercus was positive.Eleven nodules were found in the liver tissue by gross examination.Eosinophil infiltration,local fibrosis,fatty change and necrosis of hepatic cells could be found in the liver.This study suggested that beside muscle and brain cysticercosis,T.solium larvae could also invade the liver of primate and lead to hepatic lesions.The hepatic lesions could be diagnosed by ultrasound examination and immunology method.