非小细胞肺癌Stathmin mRNA的表达及临床意义

Expression of stathmin mRNA in non-small cell lung carcinoma and its clinical signification

目的 探讨Stathmin mRNA在非小细胞肺癌中的表达及临床意义.方法 成对收集非小细胞肺癌手术切除的癌和癌旁组织,抽提总RNA,利用半定量RT-PCR技术检测Stathmin mRNA的表达,对其与临床病理指标的关系进行统计学分析.结果 共收集癌和癌旁组织43对,癌组织中Stathmin mRNA表达的阳性率为76.7%,癌旁组织中为60.5%,癌组织的Stathmin相对表达量明显高于癌旁组织（Z=5.359,P=0.000）;无论是癌还是癌旁组织,男性中Stathmin的相对表达量明显高于女性（χ^2=5.059、5.799,P=0.024、0.016）.癌组织中,鳞癌中Stathmin的相对表达量高于腺癌（χ^2=5.821,P=0.016）、中央型明显高于周围型（χ^2=4.308,P=0.038）.结论 非小细胞肺癌中Stathmin mRNA的表达具有较高的阳性率,其中鳞癌、中央型肺癌和男性患者中增高最明显.

Objective To investigate the expression of stathmin mRNA in non-small cell lung carcinoma （NSCLC）and its clinical significance. Methods Two-steps RT-PCR was employed to detect the levels of Stathmin mRNA in cancer tissue and corresponding pericancerous tissue from 43 NSCLC patients. Results Among 43 patients stathmin mRNA was detected in 76.7% of cancer samples and 60.5% of pericancerous tissue samples. The relative expression levels of stathmin mRNA in cancer tissue were significantly higher than those in corresponding pericancerous tissues （Z=5.359, P=0.000 ）. In both cancer and pericancerous tis- sues male patients showed higher levels than females （χ^2=5.059,5.799,P=0.024,0.016）. The expression of stathmin mRNA in squamous cell carcinoma and central bronchogenic carcinoma was significantly higher than that in adenocarcinoma（χ^2=5.821, P=-0.016）and peripheral lung cancer（χ^2=4.308,P=0.038 ）, respectively. Conclusion Stathmin mRNA was expressed positively in most NSCLC patients, especially in male patients and patients with squamous cell carcinoma or central bronchogenic carcinoma.