不同部位和不同类型恶性淋巴瘤患者EBV感染情况分析

A study on the correlation between EBV and lymphoma of various types at different locations

目的 分析不同部位和不同类型恶性淋巴瘤患者EBV感染情况.方法 收集恶性淋巴瘤标本90例,其中鼻腔/鼻咽淋巴瘤30例,肠道淋巴瘤30例,浅表淋巴结淋巴瘤30例.组织病理学诊断为非霍奇金淋巴瘤（NHL）68例,霍奇金淋巴瘤（HL）22例.应用多种抗体标记T淋巴细胞（CD3、CD45RO、CD56）和B淋巴细胞（CD20、CD79α）、免疫组化染色（Supervision二步法）.采用EBV寡核苷酸探针（EBER）确定EBV在恶性淋巴瘤细胞中的存在.结果 NHL EBV感染阳性率（42.6%）略高于霍奇金淋巴瘤EBV感染阳性率（27.3%）,但差异无显著性意义（P ＞0.05）.鼻腔及鼻咽部NHL的EBV感染阳性率（66.7%）高于肠道NHL（23.3%）和浅表淋巴结NHL（25%）（P＜0.05）.肠道T细胞淋巴瘤EBV感染阳性率（50%）高于肠道B细胞淋巴瘤EBV感染阳性率（16.7%,P＜0.05）.结论 不同类型和部位淋巴瘤患者的EBV感染率存在不同.

Objective To explore the correlation between EBV and lymphoma of various types at different locations. Methods 90cases of malignant lymphoma （ML） were collected, which included 30 cases of nasopharyngeal lymphoma （16 T-cell lymphoma and 14 B-cell lymphoma）, 30 cases of enteric lymphoma （6 T-cell lymphoma and 24 B-cell lymphoma） and 30 cases of superficially located lymphoma （8 T-cell lymphoma and 22 Hodgkin lymphoma）. Among them, histologically 68cases were non-Hodgkin lymphoma （NHL） and 22 cases were Hodgkin lymphoma （HL）, respectively. Multiple markers for T-lymphocyte （CD3,CD45RO,CD56） and B-lymphocyte （CD20,CD79a） were used in two-step method of immunostaing. For detection of EBV in lymphoma cells, in situ hybridization for EBV small encoded RNA （EBER） were adopted. Results NHL is of higher positive rate for EBER than HL, however there is no significant difference between the two （P 〉0.05）. The positive rate for EBER in nasopharingeal lymphoma （66.7%） is much higher than those in enteric lymphoma （23.3%） and superficially located lymphoma （25%）. And the difference is significant （P 〈0.05）. Moreover, The difference of positive rate for EBER between enteric T-cell lymphoma （50%） and enteric B-cell lymphoma （16.7%） is significant. Conclusion This study indicates that with location-dependence many lymphomas are associated variously with EBV.