旋覆代赭汤对反流性食管炎模型大鼠白细胞介素-6及肿瘤坏死因子-α的影响

Effect of Xuanfu Daizhe decoction on IL-6 and TNF-α of reflux esophagitis rats

[目的]观察旋覆代赭汤对混合性反流性食管炎(RE)模型大鼠血浆及食管组织细胞因子白细胞介素(IL)-6及肿瘤坏死因子(TNF)-α水平的影响,揭示RE的关键病机及深入研究旋覆代赭汤治疗RE的作用机制。[方法]采用"食管十二指肠端侧吻合术"制作动物模型,大鼠随机分为正常对照组、假手术组、模型组、西药莫沙必利对照组(西药组)、旋覆代赭汤治疗组(中药组)。各组给予相应处理或用药后,通过ELISA的方法分别检测各组血浆及食管组织中细胞因子IL-6及TNF-α水平变化。[结果]中药组及西药组较模型组能明显降低RE模型大鼠食管组织中IL-6及TNF-α水平(P<0.05),中药组与西药组相比IL-6及TNF-α水平降低(P<0.05);而血浆中IL-6含量结果表明,中药组与西药组比较,差异无统计学意义(P>0.05),其余结果与食管组织结果一致。[结论]旋覆代赭汤可显著降低RE模型大鼠食管组织中IL-6及TNF-α水平,表明其对改善食管局部及外周炎症反应、改善食管下端括约肌的松弛情况等有显著效果。

[Objective]To investigate the effect of Xuanfu Daizhe decoction（XDD）on IL-6 and TNF-α levels of reflux esophagitis（RE）rats,and disclose the key pathology of RE and the mechanism of XDD in treating RE. [Methods]Rats were randomly divided into normal control group, model group, western medicine treatment group（Mosapride）,XDD treatment group. RE models in rats were induced by gastroduodenal end-to-side anastomosis. After corresponding interventions, esophagus tissues and plasma were collected to measure the IL-6 and TNF-α levels with ELISA. [Results]Compared with model group, XDD group, and western medicine group showed signifi- cantly lower levels of IL-6 and TNF-α in the esophagus tissues. The levels of IL-6 and TNF-α in the esophagus tissues in the XDD group were significantly lower than those in the western medicine group（P〈0.05）. The plasma IL-6 levels of the three treatment groups showed no significant difference. [Conclusion]XDD could decrease the levels of IL-6 and TNF-α in the esophagus tissues and plasma of the RE rats, indicating that it could alleviate the esophagus and peripheral inflammation,resulting in the improvement of the relaxation of the lower esophageal sphincter.