蜂毒肽对类风湿关节炎模型大鼠患侧关节的影响

Protective effect of melittin on animal rheumatoid arthritis

目的探索蜂毒主要成分蜂毒肽对类风湿关节炎(rheumatoid arthritis,RA)模型动物患关节的保护效果。方法以40只雄性SD大鼠为研究对象,随机分为蜂毒组(Bv)、蜂毒肽组(Me)、盐水对照组(Mn)和假模型组(Nn)。前三组在足掌部向足尖注射完全福氏佐剂诱导RA模型,Nn组相同位置注射生理盐水,之后于患侧足三里4组分别注射蜂毒、蜂毒肽、生理盐水和生理盐水治疗。定期测量关节厚度、缩足逃避反射时间。结果注射完全福氏佐剂4 d后患侧关节均出现关节肿胀,而Nn组无明显变化。观察至第12天时Me组、Bv组关节厚度明显低于Mn组,方差分析显示差异显著(P<0.05),这一趋势一直持续至实验结束。各组缩足逃避反射时间在实验开始时基本一致,但在造模后的第一次观察(第6天)就可以发现Me、Bv、Nn的缩足逃避反射时间都高于Mn组,方差分析显示差异显著(P<0.05),这一趋势一直持续至实验结束。结论通过蜂毒肽与全蜂毒对治疗RA效果的全面比较,证实蜂毒肽是蜂毒治疗RA的活性成分。

Objective To study the protective effect of melittin on animal models of rheumatoid arthritis （RA）. Methods Male SD rats were randomly divided into bee venom group （By） , melittin group （Me） , saline control group （Mn）, and sham group （Nn）. The first three groups were injected with complete Freund＇ s adjuvant in the hind foot palm to develop an RA model. Bee ven- om, melittin, saline, and saline were administered to the Zusanli acnpoint of the affected side to treat RA. Body weight, thickness of joint, withdrawal latency of the hind paw were regularly detected after modeling. Results The weight of all the rats increased with time, but not statistically significant, Four days after injection of complete Freund＇ s adjuvant, the joints were seen swollen, no signifi- cant change, however, was observed. From 12d, Me group and Bv group exhibited significantly Iower thickness of joints than Mn group until the end of the experiment, with significant difference by analysis of variance （ P 〈 0.05 ）. Withdrawal latency of the hind paw in each group was roughly the same at the beginning of the experiment, but significant difference after modeling was observed （ day 6）, with longer withdrawal latency in Me, By, Nn groups than in Mn group. Analysis of variance showed significant difference （ P 〈 0.05 ）. This continued until the end of the experiment. Conclusions Melittin is an active ingredient in bee venom treatment of RA.