用MTT比色法测定兔眼结膜成纤维细胞的增殖与存活

The Application of MTT Colorimetric Assay for Measuring the Proliferation and Survival of Conjunctival Fibroblasts

对Mosmann的MTT比色方法进行改良后,应用于测定兔眼结膜成纤维细胞的体外增殖与存活,获得满意结果。此方法具有快速、简便、精确、不需任何同位素、不受主观因素影响等优点,可代替~3H-Tdr掺入法测定成纤维细胞及其它细胞增殖,用于药物筛选等研究,值得推广。

After modifying the Mosmann's MTT colorimetric assay, we applied it to measure the proliferation and survival of rabbit conjunctival fibroblasts in vitro and got satisfying result. MTT eolorimetric assay has the advantages of rapidity, simplicity, precision, the lack of any radioisotope and not being affected by subject factors, so we can use it to replace the H-Tdr incorporation assay to measure the proliferation of fibroblasts and other cells and to study the drugs which inhibit or increase the growth of fibroblasts and other cells.