牛眼组织中的自由基测定

Detection of Free Radical. Within Normal Bovine Ocular Tissues by Electron Spin Resonance

使用顺磁共振仪,测定比较了真空低温条件下冻干的正常牛眼7种组织中的自由基含量。结果显示,从7种组织中均获得了明确的自由基信号(g因子=2.0048),但不同眼组织中的自由基含量差异颇大。每克干重的虹膜、睫状体、脉络膜、巩膜、视网膜、角膜和晶状体之自由基含量依次为1.74(±0.27)×10^(16)自旋数(N)、1.71(±0.18)×10^(16)N、1.54(±0.23)x10^(16)N、1.00(±0.09)×10^(15)、7.29(±1.43)×10^(14)N,4.45(±0.92)×10^(14)N和1.06(±0.16)×10^(14)N。

In this experiment free radical within normal bovine ocular tissues was detected using lyophilization under vacuum by electron spin resonance (ESR). The result showed that signal of free radical from the ocular tissues was obtained (g=2.0048). There were 1.74(±0.27)×10^(16)Number of spins/gram of dry weight tissue (N/G), 1.71(±0.18)×10^(16)N/G, 1.54(±0.23)×10~^(16)N/G, 1.00(±0.09)×10^(15)N/G, 7.29(±11.43)×10^(14)N/G. 4.45(±0.92)×10^(14)N/G and 1.06(±0.16)×10^(14)N/G in the iris, ciliary body, choroid, sclera, retina, cornea and lens, respectively.