藏灵菇源酸奶复合菌发酵剂对肠道菌群平衡的作用

The Effection on Enterobacteria Balance from Complex Bacteria Leaven of Tibetan Kefir

目的:探讨藏灵菇源酸奶复合菌发酵剂对实验性高胆固醇血症大鼠调节肠道菌群平衡的作用。方法:根据大鼠血清总胆固醇水平分组。高脂模型组和试验Ⅰ、Ⅱ组饲喂高脂饲料,对照组饲喂基础饲料;试验Ⅰ组灌胃脱脂酸奶复合菌发酵剂,试验Ⅱ组灌胃全脂酸奶复合菌发酵剂,对照组和模型组给予蒸馏水,连续8周。结果:对照组大鼠的粪便中乳杆菌、肠杆菌和产气荚膜梭菌的活菌数量处于平稳状态;4周后高脂模型组大鼠的粪便中乳杆菌的活菌数量极显著低于对照组(p<0.01),而6周后肠杆菌和产气荚膜梭菌的活菌数量极显著高于对照组(p<0.01);试验Ⅰ组和试验Ⅱ组大鼠粪便中乳杆菌的活菌数量分别在4周和6周开始极显著高于高脂模型组(p<0.01),而肠杆菌和产气荚膜梭菌的活菌数量极显著低于高脂模型组(p<0.01);试验Ⅰ组调节肠道菌群平衡效果优于试验Ⅱ组。结论:藏灵菇源酸奶复合菌发酵剂能够促进高胆固醇血症大鼠肠道有益菌的生长,并抑制有害菌的繁殖,具有明显调节肠道菌群平衡作用。

Objective：To investigate the regulated effection on enterobacteria balance from complex bacteria leaven from Tibetan Kefir,the experiment of hypercholesterolemia rats was applied.Methods：Rats were randomly divided four groups by the level of serum total cholesterol.Model group and experimental groupⅠ and experimental groupⅡ were fed with high lipoid feed,and control group was fed with basic feed.Experimental groupⅠ was ingested with complex bacteria leaven of skimmed acidophilous milk for 8 weeks.Experimental groupⅡ was ingested with complex bacteria leaven of whole fat milk for 8 weeks.Control and model group were ingested with water for 8 weeks.Results：The viable count of Bacillus lactis、 Enterobacteria and Clostridium perfringens in excrement was stability in control group.The viable count of Bacillus lactis in Model group was significantly decreased（p0.01） compared with control group after 4 weeks,and the viable count of Enterobacteria and Clostridium perfringens in model group were significantly increased（p0.01） compared with control group after 4weeks and 6weeks seperately.The viable count of Bacillus lactis in Experimental groupⅠand Experimental groupⅡwere significantly increased（p0.01） compared with model group after 4weeks and 6weeks seperately,and the viable count of Enterobacteria and Clostridium perfringens were significantly decreased（p0.01） compared with Model group.The regulated effection on enterobacteria balance of Experimental groupⅠ surpassed Experimental groupⅡ.Conclusions：The complex bacteria leaven of Tibetan Kefir could facilitate the growth of beneficial bacterium in rat intestinal tract,and restrain the growth of harmful bacterium,and had the obviously accommodate effection on enterobacteria balance.