雷丸多糖的提取分离及其抗氧化活性研究

Study on Extraction,Isolation and Antioxidant Activity of Polysaccharidefrom Omphalia Lapidescens

目的:研究雷丸多糖的提取、分离技术及其抗氧化活性。方法:用纤维素酶法酶解后对雷丸多糖进行提取工艺研究。通过Sevage法除蛋白,乙醇沉淀,活性炭脱色,再经Sephadex G-200柱层析法对其进行纯化,得到雷丸多糖OL1、OL2和OL3,并对其进行了抗氧化活性的研究。结果表明:酶解雷丸多糖的最佳提取工艺条件是:酶解温度40℃,酶添加量0.08%,酶解时间90min。结论:纤维素酶法可大大缩短雷丸多糖的提取时间,是极具应用前景的提取方法。雷丸多糖具有较强的清除自由基能力,是天然抗氧化剂的良好来源,可以进一步开发利用。

Objective：To investigate the extraction and purification of Omphalia lapidescens polysaccharide and its antioxidant activities.Methods：The O.lapidescens polysaccharide was extracted after enzymolysis by cellulase,through alcohol fractional precipitation,free proteins and colors were removed by Sevage method and charcoal,respectively.OL1、OL2 and OL3 were isolated and purified by Sephadex G-200 column.The best condition for enzymatic hydrolysis of O.lapidescens polysaccharide was studied：adding amount of cellulase 0.08%,enzymatic hydrolysis for 90 min at 35 ℃.Results：The extraction time was reduced greatly by cellulase enzymatic hydrolysis method,which indicates that it is a potential method for extraction polysacchatides from O.lapidescens.Omphalia polysaccharide is a kind of good source for natural antioxidants,has relatively strong antioxidant activities and worth further development and utilization.