1株产细菌素植物乳杆菌的筛选及所产细菌素的理化性质分析

Study on Screening of Lactobacillus Plantarum for Producing Bacteriocinand its Physicochemical Characteristics

从浙江省生产的一种火腿制品中分离筛选到1株具有抑菌活性的乳酸菌菌株LH-09,对单核细胞增生李斯特菌ATCC54003的生长具有良好的抑制作用。16SrRNA序列同源性分析鉴定乳酸菌LH-09为植物乳杆菌。在排除有机酸、过氧化氢的干扰后,确定该抑菌物质为蛋白类物质,即细菌素。理化性质分析表明细菌素LH-09具有较好的热稳定性、酸碱稳定性,可被人体内蛋白酶降解。N-羟甲基甲基甘氨酸-SDS-PAGE电泳确定细菌素LH-09的分子质量在2～6kDa之间。

Strain LH-09,isolated from ham products in Zhejiang province,produced antimicrobial substance which was inhibitory to Listeria monocytogenes 54003.Strain LH-09 was identified as Lactobacillus plantarum by using 16S rRNA gene sequence homology analysis.After eliminating the effect of organic acid and hydrogen peroxide,the antimicrobial substance with its proteinaceous nature was confirmed as bacteriocin.Bacteriocin LH-09 was heat-stable and remained activity after incubation at a wide pH.It could be degraded by proteases existing in the human body.Tricine-SDS-PAGE showed that its molecular weight was between 2-6 kDa.In this paper a preliminary study on the bacteriocin producted by Lactobacillus plantarum had been made to lay the basis for the development of natural food preservative.