肌基膜管植入对大鼠脊髓半横断损伤模型的血管化作用

Effects of muscle basal lamina transplantation on vascularization of hemisected spinal cord model in rats

目的:观察肌基膜管移植治疗大鼠脊髓损伤的血管生成情况,为在脊髓损伤中应用肌基膜管作为组织工程支架提供理论和实验依据。方法:利用冻融法将大鼠骨骼肌制备成肌基膜管,将其移植入大鼠脊髓半横断损伤模型中,分别于术后3、5、7、14和28 d取材,利用碱性磷酸酶染色观察各个时间点支架中血管生成情况,比较不同时间点(3、5、7、14和28 d)血管生成数量。结果:肌基膜管在移植入大鼠脊髓损伤区后,血管由脊髓和肌基膜管交界处长入肌基膜管内部,最终在肌基膜管内部形成血管网。新生血管面积比5 d组高于3 d组(P<0.05),7 d组高于5 d组(P<0.01),14 d组高于7 d组(P<0.01),28 d组高于14 d组(P<0.05或P<0.01)。结论:肌基膜管移植入大鼠脊髓半横断损伤模型中具有良好的血管化作用,有望成为修复脊髓损伤理想的组织工程材料。

Objective To observe the vascularization of muscle basal lamina after transplantated into rat spinal cord injury,and to provide theoretical and experimental basis for the use of muscle basal lamina as tissue engineering material in spinal cord injury.Methods Muscle basal lamina was made by the frozenthawed process and transplanted into the rat hemisected spinal cord model.The rats were sacrificed on the 3,5,7,14 and 28 day after the operation.The vascularization of scaffolds was observed at different time points by alkaline phosphatase staining（APS）.The number of blood vessels in scaffold was compared at different time points（3,5,7,14 and 28 d）.Results After transplantation,the newly formed blood vessels could grow into the muscle basal lamina scaffold from the juntional area between the scaffold and spinal cord to the inside of scaffold,and a network in the scaffold was formed.The percentage of blood vessel area increased gradually and the differences between each two groups were statistically significant,5 d 3 d（P0.05）;7 d 5 d（P0.01）;14 d7 d（P0.01）;28 d14 d（P0.01）.Conclusion Muscle basal lamina has better vascularization after transplantation into the rat spinal cord injury model and it is expected to become suitable tissue engineering material to repair spinal cord injury.