短期睡眠剥夺对大鼠外侧缰核内氨基酸类神经递质水平及c-Fos表达的影响

Effects of sleep deprivation on amino acids neurotransmitters and c-Fos protein expression in lateral habenular nucleus in rats

目的:探讨在短期睡眠剥夺(SD)情况下缰核(Hb)神经元活动的改变和Hb对睡眠的调节作用,阐明外侧缰核在SD后睡眠反弹过程中的作用。方法:将24只健康Wistar大鼠随机分为对照组和SD组,每组各12只。采用单个小平台水环境法制作SD模型,大平台组作为压力对照。高效液相-紫外检测器测量Hb内谷氨酸(Glu)和γ-氨基丁酸(GABA)水平,免疫组织化学方法测定c-Fos阳性神经元的数量。结果:与对照组比较,SD大鼠Hb内Glu水平明显增加(P<0.05),GABA水平有增加趋势,外侧缰核c-Fos阳性神经元数量增加(P<0.05),内侧缰核c-Fos阳性神经元数量无明显改变。结论:外侧缰核参与短期SD后的睡眠反弹过程,内侧缰核并没有明显作用。

Objective To investigate the changes of neuronal activity of habenula and the regulatory effect of Hb on sleep under the condition of short-time sleep deprivation（SD）,and to clarify the effects of lateral habenula（LHb） on sleep rebound after short-time SD.Methods 24 adult healthy Wistar rats were randomly divided into control group（n=12） and SD group（n=12）.The model of SD was set up by small platform surrounded by water.The typical control for this procedure was to maintain the animal on a large platform.The levels of glutamic acid（Glu） and γ-aminobutyric acid（GABA） in Hb neurons were assessed by HPLC-UV detector.The amounts of c-Fos positive neurons were detected by immunohistochemistry.Results Compared with control group,the levels of Glu in Hb was increased（P0.05）,and the level of GABA had an incrensing trend.The amount of c-Fos positive neurons was increased markedly in LHb（P0.05）,but not in medial Hb（MHb）.Conclusion The LHb is related to the sleep rebound after SD,but not the MHb.