重组人乳铁蛋白对Tca8113细胞中促炎性反应细胞因子IL-6和IL-8蛋白表达的影响

Effects of recombinant human lactoferrin on expressions of pro-inflammatory cytokine IL-6 and IL-8 in Tca8113 cells

目的:研究重组人乳铁蛋白(rhLF)对口腔鳞癌Tca8113细胞中促炎症反应细胞因子IL-6和IL-8表达的影响,探讨其抑制肿瘤细胞生长的可能机制。方法:体外培养口腔鳞癌Tca8113细胞,用rhLF处理体外培养的Tca8113细胞,应用酶联免疫吸附试验、免疫组织化学方法检测50 mg·L-1rhLF和100 mg·L-1rhLF对Tca8113细胞分泌的IL-6和IL-8蛋白表达的影响。结果:随着rhLF浓度的增加,培养上清中的IL-6和IL-8含量逐渐下降;100 mg·L-1rhLF组培养上清中IL-6和IL-8水平明显下降,与正常对照组比较差异有统计学意义(P<0.05)。免疫组化染色,IL-6、IL-8在Tca8113细胞有表达,与对照组比较,50 mg.L-1rhLF组IL-6、IL-8在Tca8113细胞的表达明显下降,表现为弱表达或不表达。结论:rhLF在体外可以特异性地抑制口腔鳞癌Tca8113细胞中IL-6和IL-8的生成,诱导肿瘤细胞炎症性环境的改变,达到抑制肿瘤生长的目的。

Objective To study the effects of recombinant human lactoferrin（rhLF ） on the expressions of pro-inflammatory cytokine IL-6 and IL-8 in Tca8113 cells in oral squamous cell carcinoma and to explore the possible mechanism of inhibiting the growth of tumor cells.Methods The Tca8113 cells cultivated in vitro were treated with rhLF（50 and 100 mg·L-1）.Enzyme linked immunosorbent assay and immunohistochemical method were used to detect the influence of rhLF on the expressions of IL-6 and IL-8 proteins.Results The levels of IL-6 and IL-8 in supernatant were gradually decreased with the increasing of the concentrations of rhLF.Compared with normal control group,the levels of IL-6 and IL-8 in 100 mg·L-1 rhLF group were significantly decreased （P0.05）.The results of immunohistochemical staining showed that the expressions of IL-6 and IL8 were detected in Tca8113 cells.Compared with control group,the expressions of IL-6 and IL-8 in 50 mg·L-1rhLF group were significantly decreased,there was only weak expression or no expression in this group.Conclusion rhLF can specificially inhibit the generation of IL-6 and IL8 in Tca8113 cells and induce the changes of inflammatory environment of tumor cells to inhibit the growth of tumor.