RP-HPLC同时测定石胆草中4种主要成分的含量

A Reversed-phase High Performance Liquid Chromatography(RP-HPLC) Procedure for Determination of Four Main Components in Boea hygrometrica(Bunge) R. Brown

目的运用反相高效液相色谱法（RP—HPLC）同时测定石胆草药材中一个苯乙醇苷成分（PGs）和3个黄酮碳苷成分（FC—GSI、FC-GSⅡ、FC—Gsm）的含量。方法研究优化了石胆草药材中主要成分的检测波长和色谱分离条件等，采用ZORBAXSB—C18（4．6mm×250mm，5μm）色谱柱，以乙腈一四氢呋喃（10：1）-0．02％磷酸为流动相，梯度洗脱，检测波长为332nm，石胆草中4个主要成分得到分离（Rs〉1．5），历时45min。结果4种成分线性关系良好，r均大于0．9990，线性范围分别为0．4～3．2μg（PGs）、0．32～2．56μg（FC—GsI）、0．4～3．2μg（FC—GsⅡ）、0．46～3．52μg（FC—GsⅢ），方法平均加样回收率〉98．9％，相对标准偏差RSD〈2．08％（n=6）。结论此方法简便、快速、准确，适合用于石胆草药材的质量控制。

OBJECTIVE To develop a reversed-phase high-performance liquid chromatography （RP-HPLC） procedure for simultaneous determination of one phenylethanoid glycosides （PGs） and three flavonoid C-glycosides （ FC-GsⅠ , FC-GsⅡ and FC-GsⅢ ） in Boea hygrometrica （Bunge） R. Brown. METHODS The detection wavelength and chromatographic separation condition were opti- mized. ZORBAX SB-C18 column （4. 6 mm × 250 mm,5 μm） was used. The 4 main components were separated with acetonitrile： tetrahydrofuran （ 10：1 ） - 0. 02% phosphate in 45 min with good resolution （ Rs 〉 1.5 ）. The components were detected at 332 nm. RESULTS All correlation coefficients were greater than 0. 999 0, and the linear ranges were 0. 4 - 3.2μ （PGs） , 0. 32 - 2.56 μg （FC-Gs Ⅰ ） , 0.4 - 3.2 μg （ FC-Gs Ⅱ ） and 0. 46 - 3. 52μg （ FC-Gs m ）, respectively. The average recoveries were greater than 98. 9%, and the relative standard deviations （RSD） were less than 2. 08% （ n = 6）. CONCLUSION The method is rapid, convenient and accurate, which can be used as the quality control method for Boea hygrometrica （Bunge） R. Brown.