HPLC测定银杏达莫注射液中游离芦丁和槲皮素的含量

RP-HPLC determination of rutin and quercetin in ginkgo damole injection before hydrolysis

目的:建立反相高效液相色谱法测定银杏达莫注射液水解前芦丁、槲皮素的含量。方法:采用Zorbax Eclipse Plus C18柱(4.6 mm×150 mm,5μm),甲醇-0.4%磷酸溶液(38∶62)为流动相,流速1.0 mL.min-1,检测波长360 nm,柱温30℃。结果:芦丁、槲皮素分别在0.046675～4.6675μg、0.019927～1.9927μg之间线性关系良好,r=0.9999;平均回收率分别为98.9%,97.8%。结论:本方法有效、简便、精确,可用于银杏达莫注射液水解前芦丁、槲皮素的含量测定。

Objective：To establish an RP-HPLC method for determination of rutin and quercetion in ginkgo damole injection before hydrolysis.Method：Zorbax Eclipse Plus C18（4.6 mm×150 mm,5 μm）column was adopted with the mobile phase of methanol-0.4% phosphoric acid（38∶62） at flow rate of 1.0 mL·min-1.Detection wavelength was set at 360 nm and the column temperature was 30 ℃.Results：The calibration curve was linear within the concenteation range of 0.046675～4.6675 μg and 0.019927～1.9927 μg respectively.The recovery of rutin and quercetion total flavone was 98.9% and 97.8%.Conclusian：The method is effective,simple and accurate,and can be used to determine the content of ginkgo leaves extract before hydrolysis in ginkgo damole injection.