降解菌HQ-C-01对克百威污染土壤的生物修复

Bioremediation of Carbofuran-contaminated Soil by Degrading Fungi Stain HQ-C-01

在室内模拟条件下，研究了降解菌HQ-C-01（Pichiaanomala）对克百威污染土壤的修复作用及其影响因素，同时研究了克百威及该菌株对土壤微生物的影响。结果表明，克百威降解率与降解菌HQ．C．01接种量呈正相关，降解菌接种量为209×10^8CFU／g干土时。对土壤中50mg/kg克百威10天降解率达82．89％；当降解菌接种量低于10^6cvu／g干土时。降解菌对克百威的降解效果较弱。土壤含水量显著影响降解菌对克百威的降解率，含水量为600g/kg时降解效果最好，降解率达85．32％，而当含水量低于200g／kg时降解效果较差。在温度范围25℃～35℃降解菌对克百威都具有较好的降解效果。不同土壤pH值对降解菌的降解作用有显著影响，在pH值为7时，降解菌对土壤中50mg／kg克百威10天降解率达85．62％，在较低和较高pH值下，降解效果较差。克百威使用对土壤菌落结构有一定的影响，对土壤真菌具有强烈刺激作用，从而使土壤微生物群落结构发生改变，而降解菌的使用可缓解克百威对土壤微生物的影响，修复受污染土壤。

Under the lab simulated conditions, the bioremediation of Fungi Stain HQ-C-01 on carbofuran-contaminated soil and the factors affecting the degradation capacity were studied, meanwhile the influences of carbofuran and Fungi Stain HQ-C-01 on soil microflora were also investigated. The results showed that there was positive correlation between the degradation rate and the inoculation quantity. The degradation rate for 50 mg/kg carbofuran after 10 days was 82.89% with 2.09×10^8 CFU/g inoculation quantity. The degradation rate was low if the inoculation quantity was less than 106CFU/g. The effect of soil moisture on the degradation rate was obvious, the degradation rate was best （85.32%） when soil moisture was 600 g/kg, but was lower when soil moisture lower than 200 g/kg. Fungi Stain HQ-C-01 had higher degradation rate when the temperature ranged from 25℃ to 35℃. Soil pH value influenced the degradation rate significantly, the degradation rate for 50 mg/kg carbofuran afrer 10 days reached highest （85.629%） when soil pH value was about 7. Carbofuran has certain influence on soil microflora by intensively stimulating soil fungi, but the application of Fungi Stain HQ-C-01 can remit the influence and thus can remediate the contaminated soil.