益气清瘟解毒合剂拆方4法对流感病毒FM1感染小鼠肺中炎性细胞因子表达的影响

Influence of four parts of forming of Yiqi Qingwen Jidu Heji(YQQWJDHJ) to lung inflammatory cytokines of model rats infected with influenza virus FM_1

目的:动态观察益气清瘟解毒合剂拆方4法(以下简称拆方4法)对流感病毒感染小鼠肺中炎性细胞因子表达的影响,探讨上述4法抗流感免疫损伤及免疫修复的机制。方法:在亚洲甲型鼠肺适应株FM1感染小鼠后的不同时相,用ELISA法检测拆方4法干预后小鼠肺中TNF-αI,L-6I,L-1I,L-10I,FN-γ的表达变化情况。结果:FM1感染小鼠肺中TNF-αI,L-6I,L-1I,FN-γ的表达均高于正常组I,L-10的表达无变化。拆方4法均在FM1感染后3～5 d显著降低肺中TNF-α的表达。其中辛凉解表法、清热解毒法及益气法在FM1感染后各时相均明显增加肺中IFN-γ的表达。辛温解表法在FM1感染后1～3 d及第7天显著降低肺中IL-6的表达,在3～7 d降低IL-1的表达,在第3天及第7天增加IFN-γ的表达,第1天及5～7 d明显增加IL-10的表达;辛凉解表法在FM1感染后各时相明显降低肺中IL-6的表达,但明显增加IL-10的表达,在感染后第3天,可能增加肺中IL-1的表达,但在感染后第7天又降低IL-1的表达;清热解毒法在FM1感染后3～7 d显著降低肺中IL-6的表达,5～7 d明显降低IL-1的表达,在1～5 d明显增加肺中IL-10的表达;益气法在FM1感染后第1天及5～7 d显著降低肺中IL-6的表达,在3～7 d明显降低IL-1的表达,在第5天显著增加肺中IL-10的表达。结论:清热解毒法和益气法在FM1感染后第5天对肺组织免疫炎症损伤具有较强的对抗作用,有利于损伤的修复,而辛温解表法在感染后第3天、辛凉解表法在感染后第5天显示出一定的对抗肺组织免疫损伤的作用。

Objective： To observe the influence of the 4 parts of forming of Yiqi Qingwen Jidu Heji（YQQWJDHJ） to lung inflammatory cytokines of the model rats infected with influenza virus dynamically, and to discuss the mechanism of 4 parts of forming to anti-influenza immune injury and restoration. Method： At the different stages of infection with the model rats infected by FM1 influenza, expression in lung of TNF-α, IL-6, IL-1, IL-10 and IFN-γ was detected after the intervention of 4 parts of forming using ELISA method. Result： The expression of TNF-α, IL-6, IL-1 and IFN-γ of model rats infected by FM1 were higher than the control group, the expression of IL-10 did not change. The expression of TNF-α was significantly reduced in 3 to 5 days after infection. By the method of relieving superficies with acrid-cold, clearing away heat and poison and replenishing Qi, the lung expression of IFN-γ was significantly increased in the stage after infection. The method of relieving superficies with acrid-warm significantly reduced lung expression of IL-6 after infection in 1 to 3 days and on the 7th day, decreased the expression of IL-1 in 3 to 7 days, increased IFN-T expression on the 3rd day and the 7th day, and significantly increased the expression of IL-10 on the 1st day and in 5 to 7 days. The method of relie- ving superficies with acrid-cold reduced the expresssion of IL-6 after infection, and significantly increased the expression of IL-10. It could increase the expression of IL-1 after infection on the 3rd day, but reduced IL-1 expression after infection 7 days. The method of clearing away heat and poison reduced lung IL- 6 expression after infection in 3 to 7 days significantly, decreased the expression of IL- 1 in 5 to 7 days, also increased the lung expression of IL-10 in 1 to 5 days significantly. The method of replenishing Qi significantly reduced the expression of IL-6 after infection on the 1st day and in 5 to 7 days, decreased the expression of IL-1 in 3 to 7 days, also significantly increased the lung IL-10 on the 5th day after infection. Conclusion： The method of clearing away heat and poison and replenishing Qi could be against the lung immune inflammatory damage and repair damage. The method of relieving superficies with acrid-warm demonstrated some immunity against lung injury on the 3th day after infection and the method of relieving superficies with acrid-cold demonstrated some immunity against lung injury on the 5th days after infection.