针刺对多发梗死性痴呆模型大鼠脑血流量的影响

Effect of Acupuncture on Cerebral Blood Flow of Multi-infarct Dementia Model Rats

目的观察"益气调血,扶本培元"组穴针刺法对多发梗死性痴呆(MID)模型大鼠脑血流量(CBF)的影响,探讨针刺治疗血管性痴呆(VD)的可能机制。方法将80只Wistar雄性大鼠随机分为正常组(10只)、假手术组(10只)、栓子造模组(60只)。栓子造模组大鼠通过右侧颈外动脉缓慢注入栓子盐水溶液0.3ml造模,7d后采用Morris水迷宫实验筛选出MID模型大鼠,并将造模成功的30只MID模型大鼠随机分为模型组、针刺组、非穴组各10只。针刺组取膻中、中脘、气海、双侧足三里和血海进行针刺;非穴组取双侧胁下非穴处进行针刺;其余组采用相同的捉抓刺激。针刺21d后运用经颅多普勒脑血流测定仪检测各组大鼠注入麻醉剂10、30、50min后脑皮层的CBF。结果模型组大鼠麻醉后各时间点脑皮层CBF均明显低于正常组(P<0.01);针刺组和假手术组大鼠麻醉后各时间点CBF均明显高于模型组(P<0.05或P<0.01);针刺组大鼠麻醉后各时间点CBF亦明显高于非穴组(P<0.01)。结论 MID模型大鼠脑皮层CBF降低,"益气调血,扶本培元"组穴针刺法可增加MID模型大鼠CBF,其可能是治疗VD的作用机制。

Objective To observe the regulative function of acupuncture with the ＂tonifying qi and regulating blood,strengthening the root and reinforcing the source＂ points on the cerebral blood flow（CBF） of multi-infarct dementia（MID） model rats to explore the possible mechanism of acupuncture treatment for vascular dementia（VD）.Methods Eighty Wistar male rats were randomized into normal group,10 rats,sham-operation group,10 rats,and emboli modeling group,60 rats.The rats of the emboli modeling group were made models of MID with the slow injection of blood emboli solution 0.3ml from the right external carotid artery.Seven days later,the MID rats were assessed by the test of Morris water maze,then 30 MID model rats were randomized into model group,acupuncture group,and non-point group,with 10 in each.The acupuncture group was treated with acupuncture at Shanzhong（CV 17）,Zhongwan（CV 12）,Qihai（CV 6）,and both sides Zusanli（ST 36） and Xuehai（SP 10）.The non-point group was treated with acupuncture at the non-points in the hypochondriac region of both sides.The other groups were given the same stimulation by catching.After treatment for 21 days,the CBF of cerebral cortex of all groups was examined with transcranial Doppler rheoencephalograph 10min,30min,and 50min after the anesthetic injection.Results In the model group,the CBF of cerebral cortex at all time points was significantly lower than in the normal group（P0.01）.In the acupuncture group and sham-operation group,the CBF at all time points was significantly higher than in the model group（P0.05 or P0.01）.And after anesthesia in the acupuncture group,the CBF at all time points was also significantly higher than in the nonpoint group（P0.01）.Conclusion Acupuncture at ＂tonifying qi and regulating blood,strengthening the root and reinforcing the source＂ points can increase the CBF of MID model rats,which might be the mechanism of the method on VD.