摘要
目的观察普罗布考对阿霉素致肾小球硬化大鼠肾组织转化生长因子β(1TGF-β1)的影响。方法建立单侧肾切除加重复注射阿霉素诱导的肾小球硬化大鼠模型,分为模型组和治疗组,设假手术组为对照组。检测各组大鼠第0、4、8、12周尿蛋白排泄量,并于12周末测定各组大鼠血清肌酐、尿素氮及血清白蛋白;行肾脏病理学检查计算肾小球硬化指数;应用免疫组化方法检测肾组织中TGF-β1的表达。结果与模型组相比,普罗布考治疗组大鼠24 h尿蛋白定量明显减少,血清肌酐、尿素氮及血清白蛋白等指标均有不同程度的改善(P<0.05),肾小球硬化程度减轻(P<0.05),肾小球内TGF-β1沉积明显减少(P<0.05)。结论普罗布考对阿霉素致肾小球硬化大鼠肾脏有部分保护作用,其机制可能与抑制TGF-β1的表达有关。
Objective To study the effect of probucol on the expression of transforming growth factor-β1 （TGF-β1） in the kidney of rats with adriamycin induced glomerulosclerosis. Methods Unilateral-nephrectomized and adriamycin-induced glomerulosclerosis rat models were established and randomly divided into model group and probucol-treated group ,while rats underwent sham-operation were served as the normal control group. At O, 4,8,12 weeks, the 24-hour urinary protein of the rats from each group were measured. After 12 weeks, serum creatinine （SCr）, blood urea nitrogen （BUN） and serum albumin （ALB） were measured. The renal tissue was observed by light microscopy to calculate gloroerulo-sclerosis index （GSI）. The TGF-β1 expression in renal tissue were detected by immunohistochemistry. Results Compared with the glomerulosclerosis model group,24 h excretion of urinary protein was obviously decreased,and indicators such as SCr,BUN, ALB and GSI were also improved in the probucol- treated group （P 〈 0.05 ）. Besides, the expression level of TGF-β1 in renal tissue was sig- niticantly lower than that in the model group （P 〈 0.05 ）. Conclusion Probucol has renoprotective effect on rats with adriamycin indnced glomeruloscleclersis and this might be associated with the inhibition of TGF-β1 expression in the kidney.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2011年第10期888-890,共3页
Journal of China Medical University
基金
辽宁省教育厅高校科研计划(L2010581)
